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Date Published
QIAprep Spin Miniprep Kit Works Perfectly
(1)
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January 31, 2023
Used to purify and isolate plasmids from a culture suspension, this kit worked great. Running 12 samples, it took less than 40 minutes to ...
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Great to Identify Exogenous Tf
(1)
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MilliporeSigma
January 30, 2023
I used this to identify exogenous Tf in a solution. Importantly, this is holo-Tf (iron bound). Since I study differences between apo- and ...
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Great His Tag Antibody for Western Blot
(1)
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Abcam
January 30, 2023
Used to determine the efficiency of His-tag protein transfection. It gives multiple bands, but there are other proteins with his tags. Used ...
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RIPA is an Efficient and Ready to Use Cell Lysis Buffer
(1)
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MilliporeSigma
January 30, 2023
We use RIPA Buffer as a reagent for cell lysis when preparing cells lysate for protein quantification. This product is very useful as is ...
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Good Nuclear Control Antibody
(1)
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Proteintech Group Inc
January 30, 2023
Lamin A/C antibody was used as a nuclear protein control. Results in HKE293 cells showed that Lamin A/C was only detected in nuclear sample...
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RAPIDstain Works Better than Coomassie
(1)
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G Biosciences
January 30, 2023
This is a great protein stain! It is more sensitive than Coomassie (the standard) and doesn't take that much more time. I used per the ...
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Great Thy1.2(CD90.2) Antibody for Flow Cytometry
(1)
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BD Biosciences
January 30, 2023
The nutritional composition of diet fundamentally modulates core physiological systems, including whole-body metabolic programs and the ...
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Best Universal Blocking Buffer for Western Blot
(1)
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Bio-Rad
January 27, 2023
This is the best blocking buffer that I have had the pleasure of using for my western blots. I first started with 5% milk in TBST, but ...
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Bioactive Hepcidin Peptide from Peptide Institute
(1)
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Peptide Institute Inc.
January 27, 2023
Hepcidin peptides can be tricky because not all are bioactive. This one is. We confirmed this by demonstrating that hepcidin reduces ...
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Always Reliable, Easy to Perform on MinElute PCR Purification Kit
(1)
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January 27, 2023
This kit allows purification of up to 5 µg PCR products or DNA in low elution (from 10 to 200ul) volumes with or without vacuum. Please ...
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Say Goodbye to Sore Thumbs with Thermo Fisher's Wellwash Microplate Washer
(1)
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Thermo Fisher Scientific
January 27, 2023
Our lab purchased this well washer because of a high workload of plate based assays to be run (ELISA, ELISPOT). Set up was fairly ...
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Good SAPK/JNK Antibody for Western Blot
(1)
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Cell Signaling Technology
January 27, 2023
The antibody was diluted with TBST 1:1000 (v/v) and the membrane was incubated overnight at 4 °C. Fibroblast (MRC-5, human cells line) were ...
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Great Thy1.1 Antibody for Flow Cytometry
(1)
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BioLegend
January 27, 2023
The nutritional composition of diet fundamentally modulates core physiological systems, including whole-body metabolic programs and the ...
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ProteinTech's Ubiquitin Antibody Is My New Go-To
(1)
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Proteintech Group Inc
January 27, 2023
ProteinTech is getting a line in the acknowledgments of my dissertation for this antibody. I tried 4 different Ub antibodies (two of which ...
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Very Good GL7 PB from BioLegend
(1)
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BioLegend
January 26, 2023
Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against ...
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Sufficient Recombinant Hephaestin for Peptide Needs
(1)
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MyBioSource.com
January 26, 2023
I used this peptide to examine a novel protein-protein interaction in a cell-free system. Given that hephaestin is a transmembrane protein, ...
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ZO-1 Antibody from Thermo Fisher Works Well
(1)
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Thermo Fisher Scientific
January 26, 2023
I used this to measure ZO-1 levels in iPSC-derived endothelial cells. The antibody worked fine, but we didn't see a difference of note.
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Nutrient-Rich Microbial Broth
(1)
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Corning Life Sciences
January 26, 2023
In our laboratory we use the Miller's LB microbial broth for maintenance and propagation of Escherichia coli (E.Coli). It works with DNA ...
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Sensitive Substrate Saves Time
(1)
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Thermo Fisher Scientific
January 26, 2023
The SuperSignal™ West Dura Extended Duration Substrate is a very sensitive Chemiluminescence Substrate for detection of HRP-coupled ...
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High-Precision Determination of Protein Concentration
(1)
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Thermo Fisher Scientific
January 26, 2023
In our laboratory we use the Pierce BCA Protein Assay Kit for the determination of the protein concentration in the detection range of 20-2...
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Good CXCR3 Antibody for Immunofluorescence
(1)
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Novus Biologicals, a Bio-Techne Brand
January 25, 2023
The role of endothelial CXCR3 in neuroinflammation was examined with CXCR3 KO mice. The expression of CXCR3 on brain endothelial cells from ...
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Reagent Works Great for Transfections
(1)
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January 25, 2023
I used this to transfect my cells with a custom plasmid to produce an HA-tagged version of a protein. The reagents worked great. I was able ...
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ELISA Works Great and is Very Specific
(1)
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IBL- America
January 25, 2023
I was surprised at how well this ELISA worked. The method was as simple as most ELISAs are. What was striking is how specific it is. In ...
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Ideal Kit for Extracting Nuclear and Cytoplasmic Protein
(1)
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Thermo Fisher Scientific
January 25, 2023
The kit was used to extract nuclear and cytoplasmic protein in cultured HEK293 cells. The protocol is very easy to follow up, and the ...
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Not My First Choice for coIP
(1)
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January 25, 2023
I used these dynabeads to attempt coIP. However, I ran into several troubleshooting problems. The main issue I had was that the antibody I ...
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"Super Sensitive" is in the Substrate's Name
(1)
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Thermo Fisher Scientific
January 25, 2023
I use this for western blot detection. It is more expensive than other ECL reagents, but the price is worth it. My peers expose their blots ...
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S6 Ribosomal Protein Antibody Works Well for Western Blot
(1)
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Cell Signaling Technology
January 24, 2023
The cells (macrophage) were seeded for 24 hours in 10% FBS RPMI. The following day were stimulated with tgf-b (5 ng/ml) for 24 hours in ...
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Great T Cell Antibody for Flow Cytometry
(1)
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BioLegend
January 24, 2023
T cell infiltration into the brain during respiratory infection in mice was measured by staining for CD3 and analyzed by flow cytometry.
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First Recommendation for coIP
(1)
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January 24, 2023
I used these beads to perform coIP in cell lysates. I like these magnetic beads because they do not use an antibody to capture your protein ...
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Good Anti-CTLA-4 Antibody in Humans
(1)
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BioLegend
January 24, 2023
Our research aims to understand how some CD4 T cells become autoreactive in melanoma patients treated with check point inhibitors. In this ...
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Great Mouse Anti-Human IgA1-PE from SouthernBiotech
(1)
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SouthernBiotech
January 24, 2023
We are an immunotherapy focused group, with interest in CD8 T-cells and their TCRs. For our experimental purposes, we were interested in ...
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HA Tag Antibody from ProteinTech Never Fails
(1)
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Proteintech Group Inc
January 24, 2023
At this rate, ProteinTech is going to get a line in the acknowledgments of my dissertation. I have never had a bad antibody from them. This ...
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Excellent Antibody to Detect Collagen I
(1)
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Abcam
January 23, 2023
We used this antibody to detect Collagen I levels in mammalian cell lysates. The antibody worked really well. The antibody is very specific....
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Amazing Technique to Show Protein Interactions
(1)
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MilliporeSigma
January 23, 2023
I love this kit and technique to show protein-protein interactions. It formed the basis of my most recent publication. The kit is very easy ...
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Reliable and Strong CD56 for Human NK Cells
(1)
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BioLegend
January 23, 2023
We work on NK subpopulations of the human immunosystem. We use this antibody in coupled with different fluorophores to identify the whole ...
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Good X-treme Gene 9 DNA Transfection Reagent from Roche
(1)
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Roche
January 23, 2023
We are an immunotherapy focused group, with interest in CD8 T-cells and their TCRs. For our experimental purposes, we routinely transduce ...
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Great Hoechst 33342 Solution for Live Cell Staining
(1)
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Thermo Fisher Scientific
January 23, 2023
Used this product at a final concentration of 1mg/ml, to stain the nuclei of living cells during imaging.
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Anti-TDP43 Antibody for Western Blot
(1)
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Abcam
January 23, 2023
We have used this antibody to detect TDP43 levels in mammalian cell lysates. The antibody is very specific and works well at the suggested ...
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Great Phospho-p44/42 MAPK Antibody for Western Blotting
(1)
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Cell Signaling Technology
January 20, 2023
Used this antibody as a MAPK signalling downstream of the EGF receptor in MCF10a cells. The antibody has very high signal so use quite ...
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Great Caveolin-1 Antibody for Flow Cytometry
(1)
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Cell Signaling Technology
January 20, 2023
Changes in Caveolin-1 expression brain endothelial cells in response to respiratory infection was measured by flow cytometry.
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Recommended RAS Antibody from CST for Western Blot
(1)
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Cell Signaling Technology
January 20, 2023
Epithelial cells were seeded in 10 % FBS F-12 k. The day after the medium was replaced with 0.5% FBS and stimulated with PDGF (10 ng/ml) for...
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Great RNase Away from Thermo Scientific
(1)
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Fisher Scientific
January 20, 2023
Works great to wipe surfaces to eliminate RNase when working with RNase sensitive samples. We use this when working with RNA extractions and...
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Easy Cloning with SOC Outgrowth Medium
(1)
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New England Biolabs
January 20, 2023
I use SOC Outgrowth Medium to allow my bacteria to recover and give them time to express the antibiotic gene resistance after transformation...
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Great Kit for Detecting LDH Activity in Samples
(1)
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January 20, 2023
We used this kit to measure LDH activity in the mammalian samples to quantitate cell death after specific drug treatment.
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Decent lgBiT-Antibody for Western Blot
(1)
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Promega
January 19, 2023
This monoclonal mouse antibody against the large subunit of the Promega-developed split-luciferase NanoBiT works well for detection via ...
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Reliable and Easy Option to Stain for TNF-a in Human
(1)
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BioLegend
January 19, 2023
Our research aims to understand how some CD4 T cells become autoreactive in melanoma patients treated with check point inhibitors. In this ...
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Easy Plasmid Purification
(1)
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January 19, 2023
I use this kit to perform plasmid DNA maxiprep. I obtain high-quality DNA that can be used for transfection.
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Cleaved PARP Antibody for Western Blot
(1)
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Cell Signaling Technology
January 19, 2023
I used this antibody diluted in TBST 1:1000 (v/v) and I incubate overnight at 4 °C. The cells (fibroblast) were induced with tgf-b for 24 ...
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Good Gel Filtration Markers Kit
(1)
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January 19, 2023
We used the protein standards from this kit for our gel filtration experiment to calculate the protein molecular weight. In the column, ...
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Reasonably Good Antibody to Detect GFP-Tag
(1)
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MilliporeSigma
January 19, 2023
We used this antibody to detect GFP tags in western blot applications. The antibody is not highly specific and works well at the suggested ...
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Camera
Cell Analysis Equipment
Cell Sorting Equipment
Cells and Strains
Centrifuge
Chemicals and Reagents
Chromatography Equipment
Columns
Concentrator
Cooler
Counter
Cytometer
Electrophoresis Equipment
Electroporation Equipment
ELISA Kits
Enzymes
Fluorescence Spectrophotometer
Freezer
Homogenizer
Imaging Equipment
Incubator
Injector
Kits
Labels / Dyes / Stains
Ladders and Markers
Light Sources
Liquid Handling Equipment
Luminometer
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Magnets
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Master Mixes
Media
Meter
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Microscopes
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Primary Cells
Proteins
Purification Equipment
Refrigerator / Freezer
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Sample Prep Equipment
Scanners
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Shaker
Slides
Software
Sonicator
Spectrometer
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Substrates
Transfection Equipment
Vacuum Manifolds
Vortexer
Water Bath
Applications
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Western Blot
Flow Cytometry
ELISA
Immunofluorescence
Immunoprecipitation
Immunocytochemistry
Immunohistochemistry
Block/Neutralize/Inhibit
Cell Isolation
Dot Blot
ChIP
Purification
Staining
Cell Culture
DNA Purification
PCR
RNA Analysis
RNA Purification
qPCR
Clean-up
Microscopy
Cloning
RT-PCR
cDNA Synthesis
Transfection
Electrophoresis
RT-qPCR
Cell Counting
Protein Purification
Next Gen Sequencing (NGS)
Library Construction
Genotyping
Gel Imaging
Protein Assay
Reporter Assay
Cell Lysis
Multiplex
Gel Staining
RNA-Seq
Live Cell Imaging
Cell Differentiation
Ligation
Concentration
HPLC
Sequencing
Chromatography
Mass Spectrometry
Labeling
Microarray
Mutagenesis
FISH
CRISPR
TUNEL Assay
In Vivo Imaging
EMSA
Cell Fractionation
Immunoassay
In Vitro Transcription
ChIP-Seq
Northern Blot
3D Imaging
RNAi
ELISPOT
PCR Cloning
Particle Analysis
Southern Blot
Protein Modification
DNA Analysis
Expression
Cell-Based Assay
Gene Editing
In Vivo Testing
Protein Labeling
Other
Species
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Human
Mouse
Rat
Non-Human Primate
Rabbit
Sheep
Hamster
Chicken
Bovine
Canine
Porcine
Horse
Monkey
C. elegans/Worm
Insect
Amoeba/Protozoa
Bacteria/Archaea
Arabidopsis
Yeast/Fungi
Squirrel
Fish
Virus
Other Invertebrate
Xenopus/Amphibian
Drosophila
Goat
Rhesus
Tissue
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Adipose
Aorta
Artery
Bladder
Blood
Blood Vessel
Bone
Bone Marrow
Brain
Breast
Bronchus
Cerebral Cortex
Colon
Cornea
Coronary Artery
Dermis
Duodenum
Ear
Embryo
Endometrium
Epidermis
Eye
Foreskin
Gingiva
Hair
Heart
Hippocampus
Hypothalamus
In Vivo
Intestine
Kidney
Large Intestine
Liver
Lung
Lymph Node
Mammary Gland
Mouth
Muscle
Ovary
Pancreas
Peripheral Blood
Peritoneum
Placenta
Prostate
Rectum
Retina
Skeletal Muscle
Skin
Small Intestine
Spinal Cord
Spleen
Stomach
Striatum
Synovium
Tail
Teeth
Testis
Thymus
Thyroid Gland
Tonsil
Trachea
Umbilical Cord
Umbilical Vein
Uterus
Vein
Cell Type
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Adipocyte
Astrocyte
B Cell
CD8+ T Cell
Competent Cells
Dendritic Cell
Embryonic Stem Cell
Endothelial Cell
Eosinophil
Epithelial Cell
Fibroblast
Glial Cell
Granulocyte
Helper T Cell
Hematopoietic Stem Cell
Hepatocyte
HUVEC
Induced Pluripotent Stem Cell
Keratinocyte
Leukocytes
Lymphocyte
Macrophage
Mesenchymal Stem Cell
Microglia
Microvascular Endothelial Cell
Monocyte
Myoblasts
Myocyte
Neuron
Neutrophil
NK Cell
NKT Cell
Oligodendrocytes
Osteoclast
PBMC
Platelets
Progenitor Cell
Red Blood Cell
Regulatory T Cells
Skeletal Muscle Cell
Smooth Muscle Cell
Splenocyte
Stem Cell
Stromal Cell
Synoviocyte
T Cell
Thymocytes
Tumor Cells
Cell Line
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HeLa
PC3 (PC-3)
HEK293
HEK293T (HEK-293T)
CHO
Jurkat
3T3
Caco-2
HepG2
THP-1
U2OS
PC12 (PC-12)
HL60 (HL-60)
A375 (A-375)
MOLT4 (MOLT-4)
MCF-7
A431
MDA-MB-231
A549
N2a
Daudi
HCT-116
Raji
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