Cell Signaling Technology
Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP ® Rabbit mAb
4858S
N/A
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I used this antibody diluted in TBST 1:1000 (v/v) and I incubate overnight at 4 °C. The cells (fibroblast) were induced with tgf-b for 24 hours and harvested with ripa buffer.
Western Blot
Fibroblast
1:1000, 4 °C, overnight
BSA 5% TBST
1:10000
Chemiluminescence
I used this antibody diluted in TBST 1:1000 (v/v) and I incubate overnight at 4 °C. The cells (fibroblast) were induced with tgf-b for 24 hours and harvested with ripa buffer
Band is clear.
Really recommended.