R&D Systems
TGF-b1
240-B
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To induce EMT for BT20 cells, 10 ng/ml TGF-b1 was administered for incubation of 72 h. The cells were collected for fractionation using NE-PER Nuclear and Cytoplasmic Extraction Reagents (78833). The expression levels of Smad3 (cat #9523) was examined to demonstrated for the distribution of the target Smad3. The results showed that Smad3 was primarily located in nuclear fractions.
10 ng/ml treatment of BT20
BT20
Sterile technique
N/A
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