BioLegend
PE/Cyanine7 anti-human CD3 Antibody
300316
HIT3a
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Our lab uses a lot of CD3 for a variety of tests to identify T cells in humans. This antibody from BioLegend is excellent. It was easy to titrate, easy to use, and cheap/affordable.
Flow Cytometry
Human T cells
15 minute at Room Temp (18-22C)
None needed
CytoFlex cytometer (Beckman Coulter)
The results (as shown in the figure) are very clean! CD3 separates very well from the negative cells, allowing for easy identification of T cells.
N/A
Affordable, easy to use.
None
This is a good antibody and is easy to use for the immunophenotyping of human T cells.