Great Ly-6G/Ly-6C Antibody for Flow

BMS
UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody

Catalog Number:

108406

Clone Number:

RB6-8C5

The nutritional composition of diet fundamentally modulates core physiological systems, including whole-body metabolic programs and the immune system– the mammalian defense to homeostatic alteration. Ketogenic diets (KDs) are extremely high in fat and very low in carbohydrates and have been shown to dramatically rewire whole-body metabolism. The KD-induced metabolic adaptation improves many physiological processes including aging, fatty acid utilization, and glucose homeostasis. However, the effects of KDs on host immunity remain less explored1–3. Thus, the overall objective of my PhD studies will be to elucidate how KDs affect the immune response in adaptive and maladaptive contexts.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse skin sample

Primary Incubation

1:100

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

a strong signal to detect Gr-1+ neutrophil in mouse skin samples. It was a clear separation from the remaining CD45+ population.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good signal.

The Bad

N/A

The Bottom Line

Great antibody for Gr-1 staining.

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