Good LEF1 Antibody for Flow Cytometry

January 06, 2023

BlueskyReddit
Anatomy and Cell Biology
University of Illinois at Chicago
Graduate Student

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Company:

Cell Signaling Technology

Product Name:

LEF1 Rabbit mAb AF488

Catalog Number:

8490

Clone Number:

C12A5

Info:Supplier Page View Product Specs

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LEF1 transcription factor expression was analyzed by flow cytometry as an indicator of Wnt signaling activation in endothelial cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Primary mouse endothelial cells

Primary Incubation

1:200, 30 minutes on ice

Blocking Agent

TruStain FcX™ PLUS (anti-mouse CD16/32) Antibody

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Cytoflex S Flow Cytometer (Beckman Coulter)

Results Summary

LEF1 expression in fixed and permeabilized endothelial cells was detectable over isotype control and was increased with pharmacological Wnt7 activation.

DOI or PMID #

N/A

Additional Notes

FoxP3 Fix/Perm (BioLegend) used to permeabilize cells.

Image Gallery

Summary

The Good

Low dilution factor needed.

The Bad

Expensive. AF488 signal is a little dim.

The Bottom Line

Antibody worked very well to analyze LEF1 expression by flow cytometry but is expensive and a little dim.

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