Easy to Use, Affordable CD27 Antibody

January 10, 2023

Protozoa Immunology
Bernhard Nocht Institut
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

CD27 FITC

Catalog Number:

124208

Clone Number:

LG.3A10

I used this antibody to analyze the maturation from naïve to effector T cells in C57BL/6 in various experiments. Cells were isolated from different organs, for example the spleen and the brain.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

C57BL/6 splenocytes

Primary Incubation

1:200, 20 min, 4°C, in 50ul, in the dark

Blocking Agent

Fc Block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

CD27 may be expressed on many T cells and can be used to analyze maturation status of T cells. Accordingly, if analyzing multiple T cell markers, a fluorochrome not inducing a lot of spillover is preferable for this marker. I got a great resolution with this FITC antibody; at the same time, FTIC does not require a lot of compensation into other channels, maintaining resolution for other markers. I was able to use this antibody at a concentration of 1:200, allowing me to stain a large number of samples with a single tube, making the experiment cost effective. I would recommend this antibody.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works on humans and mice, cheap, easy to use.

The Bad

N/A

The Bottom Line

Works well, is cheap to purchase and can be used on many samples with a dilution of 1:200.

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