Abcam
Anti-Ki67 antibody
ab15580
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We were interested in examining the proliferation of the intestinal epithelial cells in healthy vs disease states. To do this, we stained for Ki67, a marker of cell proliferation.
Immunofluorescence
Pre-fixed frozen mouse small intestine tissue section
1:100 in 1% BSA in PBS overnight at 4 degrees
5% goat serum in 1% BSA in PBS
Goat anti-rabbit AlexaFluor488 was used at 1:200 in PBS and was incubated on the section for 1 hour at room temperature in the dark
NA
Zeiss LSM 700 confocal
The proliferating crypt cells were clearly stained with Ki67 while the epithelial cells further up the villi did not stain. Staining was also scattered about in other lamina propria cells.
None
Stains proliferating cells very clearly
None to mention
The anti-Ki67 antibody stains proliferating (or recently proliferated) cells very clearly.