Ethidium Bromide Stains DNA Very Clearly

Stanford
Pathology
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen

Product Name:

UltraPure™ Ethidium Bromide, 10 mg/mL

Catalog Number:

15585-011

Our lab runs a lot of genotyping PCRs and, to visualize the PCR products, we often use ethidium bromide.

Experimental Design and Results Summary

Application

DNA gel electrophoresis

Starting Material

Genotyping PCR product

Protocol Overview

The ethidium bromide is added to the gel solution before casting at a concentration of 1:20,000. No post-staining is necessary.

Tips

Nothing in particular (do be careful with the stock solution though, as ethidium bromide can be a carcinogen!)

Results Summary

Visualization of DNA bands is very clear

Additional Notes

None

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Summary

The Good

Easy to use and works very well for DNA visualization

The Bad

None to mention

The Bottom Line

This product is great for staining DNA during gel electrophoresis.

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