Excellent CD69 Antibody

Research
Bloodworks Northwest
Postdoctoral research fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

Anti-Mouse CD69 APC

Catalog Number:

17-0691-82

Info: View Product View Product Specs

Browse Similar Products: CD69 Antibodies

This antibody was to used to identify recently activated T cells and it worked very well.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes

Primary Incubation

1:100, 30 mins, +4c

Blocking Agent

Fc block

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

LSR II

Results Summary

Staining with the CD69 APC antibody worked well. Activated T cells up regulate CD69 and conversely, down regulate CD62L.

Additional Notes

None

Related Categories

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Summary

The Good

Good separation between positive and negative

The Bad

Slightly higher background than I would like

The Bottom Line

Worth purchasing.

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