Great Antibody!

Medicine
Northwestern University
Research assistant professor

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Alexa Fluor® 647 anti-human CD294 (CRTH2) Antibody

Catalog Number:

350104

Info: View Product View Product Specs

Browse Similar Products: CD294 Antibodies

This is a great antibody! It is bright, and the recommended dilutions for flow cytometry turned out to be the best after doing titrations. Very reliable from lot to lot as well.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMC

Primary Incubation

15 min at room temp

Blocking Agent

Fc Block from Miltenyi

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry; Red 635nm Laser

Results Summary

We used this antibody to detect and sort ILC2s from peripheral blood. The antibody gives a very clear separation between positive and negative cells, which helps us to achieve a >98% pure populations after the sort.

Additional Notes

None

Related Categories

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Summary

The Good

Works well, very reliable

The Bad

None

The Bottom Line

Highly recommend!

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