Cell Signaling Technology
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody
4370
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This antibody was tested for detection of phospho- p44/42 MAPK (Erk1/2) in mouse tissue protein lysate. Mouse tissue samples were collected in RIPA buffer, sonicated and protein extract were prepared. Equal amount of proteins were resolved in 10% SDS-PAGE and immunoblotted for pErk1/2 using this Cell Signaling antibody.
Western Blot
Mouse tissue protein lysate
1:1000 (5% BSA in TBST), Overnight at 4 degrees Celsius
5% Skimmed milk in TBST (2h)
1:4000 (5%skimmed milk in TBST), 1h at RT
None
ECL
Got highly specific bright signal for phosphor-p44/42 in mouse tissue protein extract.
Got bright and highly specific signal
Best antibody I have ever seen. I recommend to all.