Fast and Excellent Plasma DNA Isolation

Carnegie Institution for Science
Embryology
Predoc fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Epigentek

Product Name:

Epiquik Circulating Cell-Free DNA Kit

Catalog Number:

P-1064-25

We plan to identify colon cancer specific genetic mutations from patient plasma samples. DNA isolation was needed to prepare whole genome sequencing. This product was specific for DNA isolation from plasma and was used because of the low cost.

Experimental Design and Results Summary

Application

DNA isolation from plasma

Starting Material

Plasma

Protocol Overview

Follow exactly the kit instructions and final DNA elution volume is 20 ul.

Tips

Take care not to over dry the capture beads during the final elution step.

Results Summary

Circulating plasma DNA was successfully isolated and enriched between 200-1000bp.

Additional Notes

None

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Summary

The Good

Isolated plasma DNA can be directly used for next generation sequencing

The Bad

Takes more time for handling the whole process

The Bottom Line

Good product for plasma DNA isolation and low price

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