Cell Signaling Technology
p70 S6 Kinase Antibody
9202
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The aim of the experiment was to test the effect of growth factor treatment on p70 S6 kinase phosphorylation in mammalian cells. Immunodetected blot was reprobed with p70 S6 Kinase antibody (from Cell Signaling) to normalize the blot.
Western Blot
Whole cell lysate
1:1000 (5% Skimmed milk in TBST), Overnight at 4 degrees Celsius
5% Skimmed milk in TBST (2h)
1:4000 (5%skimmed milk in TBST), 1h at RT
NA
ECL
This Cell Signaling antibody showed good detection of p70 S6 kinase with few non-specific bands. Non-specific signals were very weak as compared to specific p70 S6 kinase band.
None
Got bright signal for p70 S6 Kinase
Non-specific bands with low intensity were detected
Good antibody from cell signaling technology