Cell Signaling Technology
Bim (C34C5) Rabbit mAb
2933
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The aim of the research was to determine the levels of Bim in Jurkat cells after treatment with various cytokines. Jurkat cells were treated for 24h with cytokines and Bim levels were measured by Western blot.
Western Blot
Jurkat cells
1:1000, overnight, rocking, 4 degrees
10% Milk
1:10,000 in 5% milk, 30 min
No tertiary incubation
Chemiluminescence
Clear distinct bands of all three Bim isoforms
None
Easy to optimize, All three Bim isoforms are detected
Nothing negative to comment
Great antibody for the detection of Bim by Western blot