Good Antibody

Research
Bloodworks Northwest
Postdoctoral research fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

Anti-Mouse CD8a APC-eFluor® 780

Catalog Number:

47-0081-82

Info: View Product View Product Specs

Browse Similar Products: CD8a Antibodies

We routinely use this antibody in our 8-10 color multi parameter flow cytometry analysis and the CD8a antibody conjugated to APC-eF780 works very well to identify CD8 T cells. This APC-eF780 fluorochrome is also more resistant to Fix/perm buffers compared to its counterpart APC-Cy7, which can be detected in the same channel as APC-eF780.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes

Primary Incubation

1:100, 30mins, +4C

Blocking Agent

Fc block

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

LSR II

Results Summary

Good choice of antibody when designing a T cell panel and would like to utilize the APC-Cy7/APC-eFluor780 channel.

Additional Notes

None

Related Categories

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Summary

The Good

Great antibody

The Bad

None

The Bottom Line

Recommend

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