Cell Signaling Technology
Bcl-xL (54H6) Rabbit mAb
2764
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The aim of the research was to determine the levels of Bcl-xL in Jurkat cells after treatment with various cytokines. Jurkat cells were treated for 24h with cytokines and Bcl-xL levels were measured by Western blot.
Western Blot
Jurkat cells
1:1000, overnight, rocking, 4 degrees
10% Milk
1:10,000 in 5% milk, 30 min
No tertiary incubation
Chemiluminescence
Low background, clear and distinct bands of both Bcl-xL isoforms.
None
Easy to optimize
Nothing negative to comment
Good antibody for the detection of Bcl-xL by Western blot