B Cell Marker

October 16, 2015

Internal Medicine.
Rush University Medical Center
Research Assistant

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Company:

BioLegend

Product Name:

PE/Cy7 Anti-mouse/human CD45R/B220 Antibody

Catalog Number:

103222

Info: View Product View Product Specs

Browse Similar Products: CD45/B220 Antibodies

The aim of this experiment was to detect the cytokines in mouse B cells. This antibody works well to distinguish the B cell population.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splencytes and blood

Primary Incubation

1X10^6 suspension cells were incubated with 0.5ul PE-Cy7 anti-mouse B220 in 50ul FACS buffer for 30 min at 4oC.

Blocking Agent

Fc blocking antibody CD16/CD32 0.5ul

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

NA

Results Summary

Graphs show a clear B cell population.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Works well

The Bad

None

The Bottom Line

I would recommend this antibody to friends.

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