Novus Biologicals
Anti-HIF1 alpha
NB100-134
Info: View Product View Product Specs
Browse Similar Products: HIF-1 alpha Western blot Antibodies
Our research lab is focused on understanding the role of oncogenes, such as EGFR, in the metabolism, signaling pathways, growth and metastasis of human cancers including prostate, breast, colon and lung. This antibody was used to detect HIF1a in PC3 cells through western blot analysis. This antibody was purchased from Novus biologicals and detected a band for HIF1a protein in western blot.
Western Blot
PC3 cell lysates
1:2000, overnight at 4 C
5% milk
1:3000, one hour at room temperature
NA
ECL and film
30 ugs of protein samples were used for this western blot to detect HIF1a in PC3 cell lysates. I observed a very strong band using anti-HIF1a antibody around 100KD molecular weight.
None
Works well for western blot
One of the good antibodies for HIF1a