Good Fluorescent GAPDH Antibody

December 29, 2015

Neurosurgery
Havard medical school
Postdoc

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Abcam

Product Name:

Anti-GAPDH antibody [6C5]

Catalog Number:

ab105430

I am doing numerous western blots on cell lysates and I always use this antibody as a GAPDH loading control.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell lysates

Primary Incubation

RT, 15min, 1:10000 TBST, in the dark

Blocking Agent

RT, 1h, 5% milk in TBST

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Bio-Rad fluorescein

Results Summary

Very easy and quick loading control antibody for GAPDH.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Quick, easy to use

The Bad

None

The Bottom Line

Very easy and quick loading control

Share your experience with other scientists. Write a Review! »

Join the discussion