Santa Cruz Biotechnology GR Antibody (H-300)

December 18, 2015

Biobehavioral Health
Pennsylvania State University
PhD Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology

Product Name:

Santa Cruz Biotechnology GR Antibody (H-300)

Catalog Number:

sc-8992

I bought this antibody to measure GR expression in rat brain. The specific goal was to quantify the number of GR+ neurons in the rat hippocampus and paraventricular nucleus of the hypothalamus. I chose to use this antibody because I found several papers in which the authors also used this antibody.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Rat Brain

Primary Incubation

1:500 dilution with slices incubated at 4c overnight.

Blocking Agent

2% BSA

Secondary Incubation

Biotinylated Donkey anti-rabbit IgG (Jackson Immunoresearch) at 1/4000 dilution incubated at room temp for 2 hours

Tertiary Incubation

Vectastain ABC-kit according to manufacturer's instructions

Detection

Detected with .05% DAB enhanced by .05% Ni

Results Summary

The staining came out very clear with strong specific staining.

Additional Notes

The antibody did not work well for immunofluorescence in my hands

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Summary

The Good

Easy to use with no troubleshooting required. I think that I could probably use a lower concentration than I did as well, but don't want to change my protocol now.

The Bad

I did not get very strong staining when I detected the GR primary with an Alexafluor 488-conjugated secondary

The Bottom Line

I recommend this antibody for HRP-DAB detection of GR protein in rat brain tissue.

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