Western Blot Analysis of HA Tag

Biology
University of Houston
Graduate student

Overall

Quality of Results

Ease-of-Optimization

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Company:

GeneTex

Product Name:

HA tag antibody

Catalog Number:

GT423

Our research lab is focused on understanding the role of oncogenes, such as EGFR, in the metabolism, signaling pathways, growth and metastasis of human cancers including prostate, breast, colon and lung. This antibody was used to detect HA tag of target protein expressed in HEK293T cells through western blot analysis. This antibody was purchased from GeneTex and detetcted band HA tagged protein in western blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Protein samples from HEK293 cells

Primary Incubation

1:8000, overnight at 4 C

Blocking Agent

5% milk

Secondary Incubation

1:8000, one hour at room temperature

Tertiary Incubation

NA

Detection

ECL and film

Results Summary

This antibody detects HA tagged proteins with no non-specific bands at a low concentration of antibody.

Additional Notes

None

Related Categories

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Summary

The Good

Very good antibody for western blot

The Bad

None

The Bottom Line

Best antibody for detection of HA tagged proteins

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