Novus Biologicals
IL-1 beta/IL-1F2 antibody
nb600-633
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Browse Similar Products: IL-1 beta Immunohistochemistry Antibodies
I am using the NB600-633 antibody to stain for cytokines following microglia M1 suppression using Minocycline 30mg/kg in rat brain paraffin tissue. This antibody was used to stain IL-1B in rat brain section.
Immunohistochemistry
4%paraformaldehyde fixed rat brain; 6 um paraffin sections
Incubate in Primary overnight at 4oC (1:300 dil of NB600-633 in PBS containing 1%NGS and 0.3%Triton x)
5%NGS with PBS containing 0.3% Triton x for 1 hour
Vector lab pk4000 secondary biotinylated antibody (1/2000) in PBST with 2%NGS at RT for 1 hour. Meanwhile make up ABC reagent according to kit instruction in PBST.
ABC reagent 1 hour at RT.
DAB and H2O2
Get staining that looks like cells under 200x magnification
Got specs which look like cytokine staining. Citrate unmasking is necessary to enhance staining.
Works well with citrate ph6 antigen retrieval.
Very expensive. $259 for 0.1mg
Okay, but I would try from other companies.