PE/Dazzle™ 594 anti-mouse/human CD11b

Medicine
Harvard Medical school
Post doctoral research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE/Dazzle™ 594 anti-mouse/human CD11b

Catalog Number:

101255

Mouse lungs were extracted and digested with collagenase and DNase. Cells were then stimulated with PMA + ionomycin. Cells were stained with 1 in 200 for 20 mins. The antibody was used to identify CD11b positive cells in lung homogenate

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lung tissue

Primary Incubation

1:200, 20 mins

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry/ Canto II

Results Summary

Using the antibody, there was a distinct CD11b positive cell population detected in the mouse lung sample

Additional Notes

None

Related Categories

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Summary

The Good

Easy to use and clear signal

The Bad

None

The Bottom Line

It is an easy to use antibody that delivers good results

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