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Purified anti-HA.11 Epitope Tag Antibody
901501
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This antibody was used to test the expression of a HA tagged protein overexpressed in HEK293T cells. The end goal of this experiment was to use this antibody to test the interaction of a HA tagged protein with a GFP tagged bait protein.
Western Blot
HEK293T cells
1:2000 dilution in blocking agent for 16 hr at 4 deg
5% milk powder in 1xTBS-T (0.1% Tween)
Donkey anti-mouse IgG HRP used at 1:5000 dilution in blocking agent for 1 hr at room temp
None
ECL; exposure time 3 sec
HA-tagged Evc protein was over-expressed in HEK293T cells for 24 hr. Whole cell extracts were prepared in RIPA lysis buffer and 50 µg of the lysate for loaded on a 8% Tris-Glycine gel and SDS-PAGE was performed. The gel was transferred onto a nitrocellulose membrane and blotted with HA antibody. HA-Evc runs at ~130 kDa and the antibody nicely detects the protein.
Specific signal was observed
Works wonderfully with no background issues