Fast Transfer System

Miami
MI
Student

Overall

Performance

Ease-of-Optimization

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Company:

Bio-Rad

Product Name:

Trans-Blot Turbo Transfer System

Catalog Number:

1704155

This system is used for protein transfers for western blots. The system is easy to use and when using TGX mini gels, protein transfer is completed in 3 minutes. This is great for protein transfer.

Experimental Design and Results Summary

Application

Protein Transfer

Starting Material

None

Protocol Overview

Turn on machine. Make a "sandwich" with your gel and the contents of a Bio-Rad turbo transfer pack in the cassette. Insert cassette and turn on. Wait the THREE minutes (for mini TGX gels). Take out transferred membrane and turn off machine.

Tips

Make sure to orient the gel and membrane in the correct direction.

Results Summary

This is an incredible machine. Can do what it takes over an hour to do in CAPS buffer in 3 minutes flat. Transferred membranes look as good as those from traditional methods and I believe there is less change of air bubbles with this system because you make the transfer sandwich in the cassette so you minimize the chance of introducing bubbles while handling.

Features Summary

Run 2 cassettes independently, compact, fast

Additional Notes

None

Image Gallery

Summary

The Good

Easy to use

The Bad

Expensive compared to traditional method

The Bottom Line

If one has the resources, this is a great item to have.

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