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High Performance Bio-Rad PowerPac
(1)
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Bio-Rad
January 04, 2016
Whole cell protein lysate was resolved in 7.5% SDS-PAGE at 140 V using Bio-Rad PowerPac HC. Resolved proteins in SDS-PAGE gel were assembled...
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The Best Clone for Detecting Granzyme B
(1)
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BD Biosciences
January 04, 2016
GranzymeB is expressed in cytotoxic T lymphocytes and NK cells after antigen activation. Granzyme B released by T cells causes apoptosis of ...
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Anti-SPTAN1 Antibody in Immunofluorescence
(1)
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January 04, 2016
The main goal of our research is to identify new genes that play critical roles in cancer formation and development. To characterize the ...
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Anti-Mouse Gr1 BV421 for Splenocyte Staining
(1)
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BioLegend
January 04, 2016
This product was used to identify Grf+ cells amongst splenocytes (eg neutrophils or monocytes) under different conditions (eg tumor bearing ...
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Very Useful ABC Kit for Immunohistochemistry Staining
(1)
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January 04, 2016
This elite ABC kit is based on the avidin/biotin binding system with very uniform and highly active accessing of biotinylated target. It is ...
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MTT Assay (colorimetric assay) Kit for Assessing Cell Metabolic Activity
(1)
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Cayman Chemical
January 04, 2016
To estimate the cellular metabolic activity in Caco-2 cells, MTT Cell Proliferation Assay Kit was used
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An Okay Antibody for Detecting PIAS4
(1)
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Cell Signaling Technology
January 04, 2016
PIAS4 of PIAS family is a SUMO E3 ligase. It has been implicated in many cellular processes including chromatin regulation and DNA repair. ...
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Best Biophotometer for Protein and Nucleic Acid Quantification
(1)
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Eppendorf
January 04, 2016
This Biophotometer from Eppendrof was used to measure protein concentration in whole cell lysate. For this, relative absorbance of protein ...
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Western Blot Using Anti CPXM2(N-Term)
(1)
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antibodies-online
January 04, 2016
We performed Western blot analyses of CPXM2 protein expression in cell lysates of human PBMC using the anti CPXM2(N-Term) antibody from ...
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Best Value for Money PCR Machine
(1)
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Thermo Fisher Scientific
December 30, 2015
Mouse cell line was grown, serum starved and treated with specific growth factor and checked for its effect on mRNA copy number of genes ...
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Five-Minute Apoptosis Detection Kit
(1)
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December 30, 2015
This annexin V-PE apoptosis detection kit’s detection method is based on the fact that soon after apoptosis, most cell types translocate the...
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Rapid and Efficient Isolation and Purification of RNA
(1)
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QIAGEN - Sample to Insight
December 30, 2015
Rat liver tissue was preserved in RNA later. For the RNA isolation and purification, liver tissue was homogenized and cells were lysed. RNA ...
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Cell Signaling ERK Antibody
(1)
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Cell Signaling Technology
December 30, 2015
I am investigating the role of the ERK-Pathway after extracellular vesicle treatment. I choose this product since i am also using the ...
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Western Blot Analysis of Tubulin
(1)
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Santa Cruz Biotechnology, Inc.
December 30, 2015
This antibody was used to detect tubulin in PC3 cells through western blot analysis. This antibody was purchased from Santa Cruz and ...
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Anti-KAT4 Antibody in Immunofluorescence
(1)
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Abcam
December 30, 2015
The main goal of our research is to identify new genes that play critical roles in cancer formation and development. To characterize the ...
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Western Blot Using Anti-Phospho-Akt (Ser473), Clone 6F5
(1)
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MilliporeSigma
December 30, 2015
Here, we performed Western blot analyses of phosphorylated Akt1 in cell lysates of human Podocytes using the Anti-phospho-Akt (Ser473), ...
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Okay for Basic Counting but Awful for Viability Assessment
(1)
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Thermo Fisher Scientific
December 30, 2015
We count cells to set up experiments with a consistent number of input cells. We also count cells after the experiment to assess the impact ...
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Anti-Mouse TLR3 APC for Splenocyte Staining
(1)
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BioLegend
December 30, 2015
TLR3 APC was used to identify TLR3 expressing dendritic cells among murine splenocytes in different conditions (eg tumor bearing vs non ...
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Mouse Skin CD3 Lymphocytes
(1)
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SouthernBiotech
December 29, 2015
The goal is to detect CD3 positive lymphocytes in mouse skin wounds
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A Powerful Inverted Fluorescent Microscope for Any Task
(1)
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Nikon Instruments Inc.
December 29, 2015
The Nikon Eclipse Ti inverted fluorescent microscope serves our lab's fluorescent imaging needs, from simple IF assays to completely ...
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Good Antibody to Identify CD14+ T Cells
(1)
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BioLegend
December 29, 2015
The aim of the study was to identify major leukocytes subsets in human peripheral blood. Additionally we were interested in identifying ...
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Western Blot Using Anti-Akt1/PKBa (#07-416)
(1)
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MilliporeSigma
December 29, 2015
Here, we performed Western blot analyses of Akt1/PKBa protein expression in cell lysates of human podocytes using the Akt1/PKBa antibody ...
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Good Fluorescent GAPDH Antibody
(1)
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Abcam
December 29, 2015
I am doing numerous western blots on cell lysates and I always use this antibody as a GAPDH loading control.
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Best Compact Thermocycler on the Market
(1)
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Thermo Fisher Scientific
December 29, 2015
Our lab works with mice, which involves a great deal of genotyping. We run a number of different PCR's for to detect the presence of various...
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PE/Dazzle™ 594 anti-mouse/human CD11b
(1)
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BioLegend
December 29, 2015
Mouse lungs were extracted and digested with collagenase and DNase. Cells were then stimulated with PMA + ionomycin. Cells were stained ...
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Excellent Gel Dryer
(1)
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Hoefer, Inc.
December 29, 2015
Stained polyacrylamide gels used to resolve proteins were dried in GD2000 Vacuum Gel-Drying System either for preservation or for downstream...
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Clear Staining for Peritoneal Macrophages
(1)
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BioLegend
December 29, 2015
Our work involves the characterization of mouse macrophages from the peritoneal cavity. In order to gate most effectively the peritoneal ...
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T Cell Marker in Human Tissue
(1)
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BioLegend
December 28, 2015
This antibody was used to identify the CD3 population in human tissue.
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Best Infrared Sterilizer for Hood Use
(1)
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December 28, 2015
Maintaining a sterile work environment in the hood can be challenging, especially when it comes to aspirating off liquids from plates. ...
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Alpha Tubulin for Immunofluorescence
(1)
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Santa Cruz Biotechnology, Inc.
December 28, 2015
This was an experimental project to test whether we could visualize alpha tubulin under our fluorescent microscope.
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An Excellent Centrifuge Machine
(1)
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Eppendorf
December 28, 2015
I used this centrifuge machine for centrifugation of various biological samples and isolation of RNA, DNA and plasmid. This machine provides...
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Western Blot Using Anti CPXM2 from Acris Antibodies GmbH
(1)
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December 28, 2015
We performed Western blot analyses of CPXM2 protein expression in cell lysates of HEK293 cells using the anti CPXM2 antibody from Acris ...
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Western Blot Analysis of HIF1 Alpha
(1)
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Novus Biologicals, a Bio-Techne Brand
December 28, 2015
This antibody was used to detect HIF1a in PC3 cells through western blot analysis. This antibody was purchased from novus biologicals and ...
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Good Performance of Microprocessor Based Hot Plate With Magnetic Stirrer
(1)
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Corning Life Sciences
December 28, 2015
Submerged western transferred unit was placed in Corning magnetic stirrer, with a rotation value of around 800rpm. Proteins were western ...
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Excellent Alternative to Traditional Western Blotting
(1)
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LICORbio
December 28, 2015
This system is used in conjunction with IR antibodies to perform western blots. This system allows to probe for multiple proteins at once ...
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Great TNF alpha Kit
(1)
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BosterBio
December 28, 2015
This ELISA kit worked as advertised. Wells can be removed as strips, so not everything has to be used. Great product.
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Canine Immunophenotyping Antibodies
(1)
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Bio-Rad
December 23, 2015
High expression of MHCII is associated with T cell lymphomas. This Antibody is used to detect expression of MHC II on monocytes and ...
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Best Electronic Multichannel Pipette
(1)
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Eppendorf
December 23, 2015
Repeatedly pipetting with multichannel pipettes can be extremely exhausting for your forearms, and quite tedious for a set of 96-well plates...
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Immunofluorescence with DNCIC1 Antibody
(1)
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Novus Biologicals, a Bio-Techne Brand
December 23, 2015
The main goal of our research is to identify new genes that play critical roles in cancer formation and development. To characterize the ...
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Efficient Ligation of DNA Fragments for Cloning
(1)
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December 23, 2015
This assay kit was used for joining DNA fragments into a cloning vector.
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Western Blot Using Anti-Angiotensin II Type 1 Receptor Antibody #ab18801
(1)
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Abcam
December 23, 2015
We performed Western blot analyses of AT-1R protein expression in cell lysates of human podocytes using the anti-Angiotensin II Type 1 ...
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Good System for Fast and Efficient Concentration of Liquid Samples
(1)
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December 23, 2015
Commercially ordered siRNAs were packed in invivofectamine reagent (transfection reagent) using standard protocol. One hundred microliter of...
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Pierce BCA Total Protein Quantitation Kit
(1)
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December 23, 2015
This BCA protein assay kit is a detergent-compatible formulation based on bicinchoninic acid for the colorimetric detection and ...
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GFP Antibody for Immunoprecipitation
(1)
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Thermo Fisher Scientific
December 23, 2015
Here, GFP antibody from Thermo Fisher was tested for immunoprecipitation of a GFP tag expressed in HEK293T cells.
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Licor Donkey Anti-Rabbit 800CW Antibody
(1)
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LICORbio
December 23, 2015
This secondary antibody was used to detect rabbit primary antibodies. This was used in the Li-Cor Odyssey imaging system. It is a good ...
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Easy to Use DNase Kit
(1)
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QIAGEN - Sample to Insight
December 22, 2015
This small kit comes with DNase enzyme tube and two buffer tubes. It is easy to make working solution and only need 15 min at room ...
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Anti-mouse CD103 APC for Splenocyte Staining
(1)
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BioLegend
December 22, 2015
Splenocytes were analyzed for the presence of CD103+ DC under various conditions (eg tumor bearing vs non tumor bearing mice)
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Western Blot Using Anti-Nephrin Antibody from Abcam
(1)
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Abcam
December 22, 2015
We performed Western blot analyses of nephrin protein expression in cell lysates of human podocytes using the anti-Nephrin antibody from ...
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Antibody is Non-Specific
(1)
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Novus Biologicals, a Bio-Techne Brand
December 22, 2015
We used this antibody to screen cdyl2 knockout candidates using Western Blots. The first lane shows wildtype cell lysate while the rest show...
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Western Blot Analysis of HA Tag
(1)
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GeneTex
December 22, 2015
This antibody was used to detect HA tag of target protein expressed in HEK293T cells through western blot analysis. This antibody was ...
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