Abcam
Anti-Nephrin antibody
ab58968
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We performed protein expression analyses in human podocytes regarding the generation of different factors involved in apoptosis regulation, signaling, cell structure maintenance and cell viability. Specifically phospho-specific antibodies were used to analyse the signaling activity normalized againt total protein expression (see Akt, AMPK and mTOR). We performed Western blot analyses of nephrin protein expression in cell lysates of human podocytes using the anti-Nephrin antibody from Abcam. We detected nephrin.
Western Blot
Cell lysate of human podocytes
Dilution 1:500, 16 hours, 4°C
5% skim milk powder in TBST buffer
Dilution: 1:5000, 1 hour, room temperature
ECL - 5 min, room temperature
ECL
A single band was detected after 5 min (neprin at 85 kDa; see figure).
None
A single band was detected
Difficult to optimize Ab concentration for WB
This antibody is well suited for Western blot analyses of nephrin expression in human podocytes