Anti-GFP for DAB Stain

Psychology
Beckman Institute for Advanced Science and Engineering
Undergraduate Research Assistant

Overall

Quality of Results

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Company:

Life Technologies

Product Name:

Anti-GFP, chicken lgY fraction

Catalog Number:

A10262

The aim of the research was to incorporate light activated proton pumps into the membrane of new neurons and to be able to inactivate the cells. The gene construct used EGFG as a reporter gene. The product was used to label the EGFP positive cells using DAB and a biotinylated secondary.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Coronal tissue slices of mouse brains

Primary Incubation

1:3000 dilution for 48 hours in a cold room.

Blocking Agent

Normal Goat Serum

Secondary Incubation

1:500 for 2 hours

Tertiary Incubation

DAB for 5 minutes

Detection

DAB

Results Summary

The product worked very well and the EGFP was clearly labeled after DAB administration.

Additional Notes

None

Related Categories

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Summary

The Good

The product bound well.

The Bad

The dilution factor can vary greatly between tissue samples.

The Bottom Line

This is a great product.

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