Anti-mouse CD103 APC for Splenocyte Staining

December 22, 2015

Tisch Cancer Institute
Icahn School of Medicine at Mount Sinai
Post-Doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

APC anti-mouse CD103 Antibody

Catalog Number:

350216

Splenocytes were analyzed for the presence of CD103+ DC under various conditions (eg tumor bearing vs non tumor bearing mice)

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:100, 15min, 4C

Blocking Agent

Blocking Buffer containing sera (rat, rabbit, human, goat)

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry (BD) LSRFortessa

Results Summary

Good staining of CD103 amongst splenic DC (identified by CD11c and MHCII)

Additional Notes

None

Related Categories

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Summary

The Good

Easy to identify if DCs are plenty

The Bad

Difficult to identify CD103+ DC if DC are sparse (eg homeostasis)

The Bottom Line

Good antibody to identify CD103+DC

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