Propidium Iodide Solution from BioLegend

Product Specs
ItemPropidium Iodide Solution
CompanyBioLegend
Price $54.00Supplier Page View Company Product Page
Catalog Number421301
ApplicationsFC - Quality tested
Quantity2 mL
FormatPhosphate-buffered saline, pH 7.2, containing 0.09% sodium azide
Concentration0.5mg/ml

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Immune Modulatory Oxysterols Produced from Cholesterol-Containing Lipid Nanoparticles Regulate Tumor GrowthACS NanoMarch 31, 2026Patricia Ines Back, Shadan Modaresahmadi, Jalpa Patel, Indhumathy Subramaniyan, Vindhya Edpuganti, Md. Rakibul Islam, Seonjuti Chowdhury, Adam J. Carr, Qisheng Zhang, William C. Putnam, et al.1 × 106 cells were stained with APC Annexin V (Cat. #640920, BioLegend) and propidium iodide (Cat. #421301, BioLegend). Apoptosis and necrosis controls were used to set up the gating strategies. LSR Fortessa

(2) Spatial multi-omics characterization of neuroblastoma reveals ferroptosis-associated metabolic features in high-risk tumorsGenome MedicineMarch 31, 2026Cui Tu, Chin Wee Tan, James Monkman, Ana Clara Almeida, Gabrielle Antonio-Carreon, Natacha Omer, Giaan Hull, Kimberly Chung, Ahmed M. Mehdi, Antonietta Salerno, et al.Cells were then treated with 10 µM of ML210, FIN56 or FINO2 and propidium Iodide (PI) (BioLegend, #421301) was added to assess cell viability. The plate was placed in the Sartorius Incucyte S3 live cell analysis

(3) Dose-dependent effects of mitoquinone on mitochondrial function and post-thaw quality of cryopreserved canine spermVeterinary WorldMarch 31, 2026Abbas Farshad, Emilia Diel, Axel Wehrend20°C–22°C in the dark, followed by an additional incubation with 5 μL propidium iodide (PI, Cat. No. 421301, BioLegend Inc.) for 15 min. Before analysis, samples were diluted with 400 μL binding buffer, and at

(4) Hierarchical membrane-chromatin tethering buffers nuclear envelope assembly against alterations in lipid fluxbioRxivMarch 2, 2026Sarah R. Barger, Sofia Sepúlveda, Han Yang, Marc Goudge, Shoken Lee, Neale D. Ridgway, Shirin Bahmanyarthe G1/S synchronization protocol was confirmed by flow cytometry using propidium iodide (Biolegend 421301) staining. Oleate treatment Oleic acid (Cayman Chemicals 90260) was conjugated to fatty-acid free

(5) Enhancing 5-fluorouracil efficacy in colorectal cancer by inhibiting glutathione antioxidant mechanisms with an xCT inhibitorScientific ReportsFebruary 22, 2026Senanur Malcanlı, Remzi Okan Akar, Engin Ulukaya, Ali Burak Özkaya, Öykü Gönül Geyikanalysis Hoechst 33,342 (ChemCruz CAT NO: sc-495790) and propidium iodide (PI) staining (Biolegend CAT NO: 421301) were performed to determine the effect of CB-839 and 5-FU, Polydatin and 5-FU, and IKE and 5-FU combinations

(6) Tissue-resident macrophage survival depends on mitochondrial function regulated by SerpinB2 in chronic inflammationNat CommunFebruary 12, 2026Sathish Babu Vasamsetti, Samreen Sadaf, Mohammad A. Uddin, Jixing Shen, Ebin Johny, Awishi Mondal, Jonathan Florentin, Liqun Lei, Aleef Mannan, Krithika Sudhakar Rao, et al.and stained with 5 µl annexin V Alexa Fluor 488 and 1 µl of 100 µg/ml propidium iodide (BioLegend, #421301). The cells were incubated at room temperature for 15 minutes, and 400 μl of 1x binding buffer was added

(7) Targeting immune cells in the aged brain with engineered proteins reveals that engineered cytokine IL-10 enhances neurogenesis and improves cognitionImmunityFebruary 10, 2026Paloma Navarro Navarro Negredo, Justin You, Max Hauptschein, Adam B. Schroer, Daniel J. Richard, Gita C. Abhiraman, Andy P. Tsai, Eric D. Sun, Giulia Notarangelo, Julliana Ramirez-Matias, et al.11330032 Percoll®,Sigma-Aldrich,Cat# GE17-0891-01 D-(+)-Glucose,Sigma-Aldrich,Cat# G7021 Propidium Iodide Solution,BioLegend,Cat# 421301 "Collagenase, Type IV, powder",Thermo Fisher Scientific,Cat# 17104019 "HBSS, calcium, magnesium, no phenol red

(8) Fusobacterium nucleatum manipulates host autophagy to promote its intracellular survival and treatment resistance in nasopharyngeal carcinomaMolecular cancerFebruary 4, 2026Wang JY, Lu YQ, Tan XR, Ma SS, Dai JH, Feng SY, Duan YF, Bai JW, Li YQ, Gong S, et al.subsequently resuspended in 200 µl of PBS supplemented with pre-mixed propidium iodide (PI, Biolegend, 421301), followed by incubation in the dark at room temperature for 5 min. The proportion of dead cells was

(9) DNA-PKcs controls the cytotoxic T cell response to cancer and transplant allograft through regulating LAT-dependent signalingCell ReportsJanuary 27, 2026Randall R. Rainwater, Ana C. Azevedo-Pouly, Zachary J. Waldrip, Belle H. Hicks, Nicholas A. Callais, Brian Koss, Aaron J. Storey, Lyle Burdine, Marie Schluterman BurdineAesar,Cat#A16046 flow cytometry staining buffer,Invitrogen,Cat #00-4222-26 Propidium iodide solution,Biolegend,Cat:421301 "6-0 PROLENE, bv-1",Ethicon,Cat# 8709h Protein a/g agarose beads,Santa Cruz Biotechnologies,Cat#Sc-2003 "Bovine

(10) An innovative engineered IL-10 monomer strengthens T cell-mediated anti-tumor responses through anti-PD-1 cis-deliveryCell Reports MedicineJanuary 20, 2026Ce Gu, Jian Guo, Chang Zhou, Peipei Hu, Xiaodong Wu, Jiaojiao Ding, Xinxin Zhou, Liao Zeng, Wen Yu, Yingye Ou, et al.ab282858 Biotin Rabbit polyclonal to C1q,abcam,Cat#: ab48341 Propidium iodide Solution,BioLegend,Cat#: 421301 Annexin V Binding Buffer,BioLegend,Cat#: 422201 ,, Critical commercial assays,Critical commercial

(11) Targeting PLK1 Reduces MMP10 to Enhance Radiosensitivity in HPV— Head and Neck CancerClinical Cancer ResearchJanuary 16, 2026Julianna Korns, Maria A. Lehn, Shreya Shyamsunder, Mario Medvedovic, Stephanie M. Bryant, Mathieu G. Sertorio, Maya Ridinger, Trisha M. Wise-Draper, Vinita Takiar(100ug/ml) was added to the cell pellet to ensure only DNA was stained. Propidium iodide (BioLegend, Cat# 421301) was diluted in cell staining buffer then added to the cell pellet. Unstained cells were included as

(12) A GDF-15–GFRAL axis controls autoimmune T cell responses during neuroinflammationNature ImmunologyJanuary 15, 2026Jana K. Sonner, Audrey Kahn, Lars Binkle-Ladisch, Jan Broder Engler, Beatrice Haack, Christina Zeiler, Lisa Unger, Simone Bauer, Felix Fischbach, Giovanni Almanzar, et al.followed by staining with AF647-labeled NeuN antibody and 0.25 µg ml−1 propidium iodide (BioLegend, 421301) as a DNA counterstain. NeuN+ and NeuN− single nuclei were sorted using a BD FACSAria III cell sorter

(13) Microglial TBK1 Signaling Promotes Breast Cancer Brain MetastasisCancer ResearchJanuary 2, 2026Fatima Khan, Edgar Petrosyan, Yang Liu, Atousa Bahrami, Erfan Taefi, Changiz Geula, Maciej S. Lesniak, Peiwen Chenwere harvested and stained with Apotracker at a 1:10 dilution. Propidium iodide solution (BioLegend, 421301) was then added to identify late apoptotic and necrotic cells. The signals for fluorescein isothiocyanate

(14) Mycobacterium tuberculosis peptide-specific T cells in pulmonary granulomas display broad effector functionsiScienceDecember 19, 2025Christine E. Nelson, Keith D. Kauffman, Kevin C. Osum, Shunsuke Sakai, Jay Buchanan, Jean Michel Chanchu, Melanie Cohen, Julie Laux, Iyadh Dougi, Katherine M. Barrows, et al.5% CO2 for 16 h. Rested samples were counted and stained with 5uL propidium iodide (BioLegend cat #421301) for 5 min prior to FACS sorting. PI negative cells (live) were captured, counted, and split into two

(15) SMAC mimetics sensitize HIV-infected cells to oncolytic virus-mediated deathFront ImmunolDecember 18, 2025Bengisu Molyer, Yasmeen Ameeriar, Jonathan B. Angel(0.5 μl/tube). The cell viability was measured by staining with a PI solution (0.1 μl/tube) (cat. no. 421301; BioLegend). The activity of caspase-3/7 and caspase-1 was determined using the Far-Red Fluorescent FLICA® 660 Caspase-3/7 (DEVD) Assay Kit

(16) Galectin-3 shapes microglial phenotype through endogenous and exogenous mechanismsFront Cell NeurosciDecember 18, 2025Lluís Camprubí-Ferrer, Yiyi Yang, Rosalía Fernández-Calle, Antonio Boza-Serrano, Juan García-Revilla, Javier Frontiñán-Rubio, Tomas Deierborg(WT cells). After that, propidium iodide (PI)-negative single-cells (1:1000 dilution, BioLegend, Cat: 421301, 5 min incubation) were sorted using the BD FACSAria™ III Cell Sorter (BD Biosciences) in new 96-well

(17) Ortho -Hydroxyanilides: Slow-Acting, Selective Histone Deacetylase 1/2 Inhibitors Suitable for Photocaging ApplicationsACS Pharmacology & Translational ScienceDecember 12, 2025Irina Honin, Tao Sun, Nisha Setia, Linda Schäker-Hübner, Finn K. HansenV-Pacific blue (catalog# 640945, BioLegend, San Diego, CA, USA) and 10 μL/well propidium iodide (catalog# 421301, BioLegend, San Diego, CA, USA), incubated for 15 min and measured at the Guave EasyCyte 11HT (Guava

(18) The 3D Genome Structure of a Blcap-Linked Silencer Loop Regulates Terminal Differentiation During Lens Fiber Cell DenucleationInvest Ophthalmol Vis SciDecember 8, 2025Shurui Ke, Zhong Liu, Jingyu Ma, Yingfeng Zheng, Lixia Luo5 mL tube, followed by the addition of 5 µL APC-conjugated Annexin V and propidium iodide (PI; no. 421301; BioLegend). After gentle mixing, samples were incubated in the dark at room temperature for 15 minutes.

(19) Preclinical Pharmacokinetic, Pharmacodynamic, and Safety Profile of OBI-992: A Novel TROP2-Targeted Antibody–Drug ConjugateMolecular Cancer TherapeuticsDecember 2, 2025Chi-Sheng Shia, Shih-Ni Wen, Ren-Yu Hsu, Jyy-Shiuan Tu, Hui-Wen Chang, Hao-Cheng Weng, Jhih-Jie Yang, Ming-Feng Chiang, Yu-Hsuan Tsao, Chi-Huan Lu, et al.were incubated with ADCs for 6 days, followed by CD66b and propidium iodide (PI, BioLegend, catalog #: 421301) staining. Following the CounterBright Beads (Invitrogen, catalog #: C36950) manufacturer’s instructions,

(20) Deep immunophenotyping in aneurysmal subarachnoid hemorrhage: a prospective and controlled clinical studyJournal of NeuroinflammationNovember 29, 2025Björn B. Hofmann, Dilaware Khan, Igor Fischer, Daniel Hänggi, Sajjad MuhammadFBS in PBS. To discriminate between live and dead cells, 5 µL of Propidium Iodide Solution (Cat# BLD-421301, BioLegend (Via Biozol)) was added to each sample and incubated for 4 min on ice. After that, the samples

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1/20/2021PI is a Simple but Effective live/dead Stain Anonymous ReviewTo further characterize cell populations it is best to initially gate on live cells. in this experiment I used PI as my live/dead stain.

Propidium Iodide Solution from BioLegend

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Propidium Iodide Solution from BioLegend

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