Propidium Iodide Solution from BioLegend

Product Specs
ItemPropidium Iodide Solution
CompanyBioLegend
Price $54.00Supplier Page View Company Product Page
Catalog Number421301
Quantity2 mL
ApplicationsFC - Quality tested
FormatPhosphate-buffered saline, pH 7.2, containing 0.09% sodium azide
Concentration0.5mg/ml

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Metabolic and cellular physiological differences between embryonic breast and leg muscle satellite cells in chickensPoultry ScienceJune 1, 2026Jongryun Kim, Jeongeun Lee, Dongjin Yu, Leecheon Kim, Darae Kang, Jinryong Park, Guybong Song, Hakkyo Lee, Kwanseob Shimμg/mL RNase A (70856, Sigma-Aldrich, St. Louis, MO, USA) in PBS and 20 μg/mL propidium iodide solution (421301, BioLegend, San Diego, CA, USA). The prepared samples were transferred to FACS tubes and analyzed by

(2) DKC1 promotes colorectal cancer progression and therapy resistance by dysregulating sphingolipid biosynthesisNat CommunMay 18, 2026Umar K. Khan, Ayush Goel, Shivansh Nigam, Nazia Chaudhary, Ayush Praveen, Anjan Roy, Fabeha Fatima, Dilpreet K. Sawhney, Afiya Dalwai, Akshay Shendre, et al.and fixed with 70% ethanol. Subsequently, they were stained with propidium iodide (BioLegend, Cat # 421301) as per the manufacturer’s protocol before acquisition. Cell cycle distribution was assessed using an

(3) miR-4772-3p serves as a potential diagnostic and prognostic biomarker in septic acute kidney injury by targeting CISD2Ren FailMay 17, 2026Qiang Chen, Gaoshang Zhuflow cytometry after double staining with Annexin V-FITC (640906, BioLegend, USA) and propidium iodide (PI) (421301, BioLegend, USA). HK-2 cells were harvested, rinsed with ice-cold PBS, and resuspended in binding buffer.

(4) Enhancing the Therapeutic Profile of Dapagliflozin: Chitosan Nanoparticle Encapsulation for Intestinal ApplicationsFASEB BioadvMay 14, 2026Agni Klonari, Antrea-Maria Athinodorou, Eirini Papanikolaou, Anastasia Skonta, Myrto G. Bellou, Lampros Lakkas, Konstantinos I. Tsamis, Georgios S. Markopoulos, Haralambos Stamatis, Petros Bozidis, et al.100 μL Annexin V Binding Buffer (Biolegend 422201). Annexin V (Biolegend 640906) and PI (Biolegend 421301) (5 μg/mL each) were added under dark conditions, and samples were gently vortexed and incubated at

(5) Targeting WNK1 Releases Differentiation Block in Acute Myeloid LeukemiabioRxivApril 30, 2026Jordan D. Cress, Emily M. Katoni, Parameswaran Ramakrishnanethanol for 30 minutes at 4°C then washed twice with PBS. 20 μg/mL Propidium iodide (PI) (BioLegend, 421301) and 100 μg/mL RNase (Sigma, R6148) were added to the cell suspension. Samples were incubated at room

(6) miR-99b-5p inhibition drives apoptosis and tumor shrinkage in triple-negative breast cancer: functional characterization through AGO2-RIP-seq and mechanistic insightsFrontiers in OncologyApril 22, 2026Senem Noyan, Kubra Nur Kaplan Ilhan, Muge Ocal Demirtas, Begum Karaoglu Dallı, Bora Ergin, Rabia Sen, Hakan Gurdal, Bala Gur Dedeoglu106 cells/ml. Annexin V-FITC (5 µl, 640906, BioLegend) and propidium iodide (PI) (5 µl, 0.5 µg/µl, 421301, BioLegend) were then added to the cell suspension and incubated for 15 minutes at room temperature

(7) Excessive pyroptosis mediates the exacerbation of pneumonia caused by low-lethality influenza virus and secondary MRSA co-infectionCell Death DiscoveryApril 2, 2026Zi-Chen Tian, Yang Liu, Yi-Jun Niu, Xin Ai, Su-Ya Lao, Wei-Ming Xu, Xiao-Tong Lin, Cheng-Jie Xia, Zhi-Xuan Cai, Hai-Yan Zhu, et al.Antibody (ABclonal, A27478); anti-mouse CD11b Antibody (BioLegend, Cat#101205); PI (BioLegend, Cat#421301); Annexin V-FITC (Beyotime, C1062M)] and fixed with 4% paraformaldehyde. The suspensions were then washed

(8) Dose-dependent effects of mitoquinone on mitochondrial function and post-thaw quality of cryopreserved canine spermVeterinary WorldMarch 31, 2026Abbas Farshad, Emilia Diel, Axel Wehrend20°C–22°C in the dark, followed by an additional incubation with 5 μL propidium iodide (PI, Cat. No. 421301, BioLegend Inc.) for 15 min. Before analysis, samples were diluted with 400 μL binding buffer, and at

(9) Immune Modulatory Oxysterols Produced from Cholesterol-Containing Lipid Nanoparticles Regulate Tumor GrowthACS NanoMarch 31, 2026Patricia Ines Back, Shadan Modaresahmadi, Jalpa Patel, Indhumathy Subramaniyan, Vindhya Edpuganti, Md. Rakibul Islam, Seonjuti Chowdhury, Adam J. Carr, Qisheng Zhang, William C. Putnam, et al.1 × 106 cells were stained with APC Annexin V (Cat. #640920, BioLegend) and propidium iodide (Cat. #421301, BioLegend). Apoptosis and necrosis controls were used to set up the gating strategies. LSR Fortessa

(10) Spatial multi-omics characterization of neuroblastoma reveals ferroptosis-associated metabolic features in high-risk tumorsGenome MedicineMarch 31, 2026Cui Tu, Chin Wee Tan, James Monkman, Ana Clara Almeida, Gabrielle Antonio-Carreon, Natacha Omer, Giaan Hull, Kimberly Chung, Ahmed M. Mehdi, Antonietta Salerno, et al.Cells were then treated with 10 µM of ML210, FIN56 or FINO2 and propidium Iodide (PI) (BioLegend, #421301) was added to assess cell viability. The plate was placed in the Sartorius Incucyte S3 live cell analysis

(11) Symbiotic exclusivity between CLOCK and TFPI2 drives stemness and immunosuppression in glioblastoma modelsJ Clin InvestMarch 17, 2026Fei Zhou, Lizhi Pang, Yang Liu, Fatima Khan, Peiwen Chenand stained with Apotracker (1:10 dilution). Then 5 μL propidium iodide (PI) solution (BioLegend, # 421301) was added to label the necrotic and late apoptotic cells. Cells were washed with PBS three times. Fluorescein

(12) Adhesion‐Related Macrophages Regulate Metabolic Homeostasis Through CAV‐1 DependencyAdvanced ScienceMarch 12, 2026Wanyu Hu, Xiyan Liao, Limin Xie, Xiaoxiao Sun, Luodi Jing, Qin Zeng, Hongwei Jiang, Haowei Zhang, Lei Li, Fanqi Wang, et al.Thermo Fisher Scientific). Cells were also stained with viability dyes including propidium iodide (1:200, 421301, Biolegend), Zombie NIR (1:100, 423106, Biolegend), or DAPI (0.2 µg/mL, 4083, Cell Signaling Technology).

(13) Hierarchical membrane-chromatin tethering buffers nuclear envelope assembly against alterations in lipid fluxbioRxivMarch 2, 2026Sarah R. Barger, Sofia Sepúlveda, Han Yang, Marc Goudge, Shoken Lee, Neale D. Ridgway, Shirin Bahmanyarthe G1/S synchronization protocol was confirmed by flow cytometry using propidium iodide (Biolegend 421301) staining. Oleate treatment Oleic acid (Cayman Chemicals 90260) was conjugated to fatty-acid free

(14) Enhancing 5-fluorouracil efficacy in colorectal cancer by inhibiting glutathione antioxidant mechanisms with an xCT inhibitorScientific ReportsFebruary 22, 2026Senanur Malcanlı, Remzi Okan Akar, Engin Ulukaya, Ali Burak Özkaya, Öykü Gönül Geyikanalysis Hoechst 33,342 (ChemCruz CAT NO: sc-495790) and propidium iodide (PI) staining (Biolegend CAT NO: 421301) were performed to determine the effect of CB-839 and 5-FU, Polydatin and 5-FU, and IKE and 5-FU combinations

(15) Tissue-resident macrophage survival depends on mitochondrial function regulated by SerpinB2 in chronic inflammationNat CommunFebruary 12, 2026Sathish Babu Vasamsetti, Samreen Sadaf, Mohammad A. Uddin, Jixing Shen, Ebin Johny, Awishi Mondal, Jonathan Florentin, Liqun Lei, Aleef Mannan, Krithika Sudhakar Rao, et al.and stained with 5 µl annexin V Alexa Fluor 488 and 1 µl of 100 µg/ml propidium iodide (BioLegend, #421301). The cells were incubated at room temperature for 15 minutes, and 400 μl of 1x binding buffer was added

(16) Targeting immune cells in the aged brain with engineered proteins reveals that engineered cytokine IL-10 enhances neurogenesis and improves cognitionImmunityFebruary 10, 2026Paloma Navarro Navarro Negredo, Justin You, Max Hauptschein, Adam B. Schroer, Daniel J. Richard, Gita C. Abhiraman, Andy P. Tsai, Eric D. Sun, Giulia Notarangelo, Julliana Ramirez-Matias, et al.11330032 Percoll®,Sigma-Aldrich,Cat# GE17-0891-01 D-(+)-Glucose,Sigma-Aldrich,Cat# G7021 Propidium Iodide Solution,BioLegend,Cat# 421301 "Collagenase, Type IV, powder",Thermo Fisher Scientific,Cat# 17104019 "HBSS, calcium, magnesium, no phenol red

(17) Fusobacterium nucleatum manipulates host autophagy to promote its intracellular survival and treatment resistance in nasopharyngeal carcinomaMolecular cancerFebruary 4, 2026Wang JY, Lu YQ, Tan XR, Ma SS, Dai JH, Feng SY, Duan YF, Bai JW, Li YQ, Gong S, et al.subsequently resuspended in 200 µl of PBS supplemented with pre-mixed propidium iodide (PI, Biolegend, 421301), followed by incubation in the dark at room temperature for 5 min. The proportion of dead cells was

(18) DNA-PKcs controls the cytotoxic T cell response to cancer and transplant allograft through regulating LAT-dependent signalingCell ReportsJanuary 27, 2026Randall R. Rainwater, Ana C. Azevedo-Pouly, Zachary J. Waldrip, Belle H. Hicks, Nicholas A. Callais, Brian Koss, Aaron J. Storey, Lyle Burdine, Marie Schluterman BurdineAesar,Cat#A16046 flow cytometry staining buffer,Invitrogen,Cat #00-4222-26 Propidium iodide solution,Biolegend,Cat:421301 "6-0 PROLENE, bv-1",Ethicon,Cat# 8709h Protein a/g agarose beads,Santa Cruz Biotechnologies,Cat#Sc-2003 "Bovine

(19) An innovative engineered IL-10 monomer strengthens T cell-mediated anti-tumor responses through anti-PD-1 cis-deliveryCell Reports MedicineJanuary 20, 2026Ce Gu, Jian Guo, Chang Zhou, Peipei Hu, Xiaodong Wu, Jiaojiao Ding, Xinxin Zhou, Liao Zeng, Wen Yu, Yingye Ou, et al.ab282858 Biotin Rabbit polyclonal to C1q,abcam,Cat#: ab48341 Propidium iodide Solution,BioLegend,Cat#: 421301 Annexin V Binding Buffer,BioLegend,Cat#: 422201 ,, Critical commercial assays,Critical commercial

(20) OSM-11 modulates salinity-stress tolerance in Caenorhabditis elegansbioRxivJanuary 16, 2026Pengchi Zhang, Beining Xue, Yusu Xie, Kunlin Li, Hanwen Yang, Peiqi Sun, Liusuo Zhangdetailed methods see Section 7. Propidium Iodide (PI) staining 2.5 μl of 0.5 mg/mL PI (BioLegend, 421301) were added into 50 μl OP50 bacteria to prepare the food mixture42. For detailed methods see Section

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1/20/2021PI is a Simple but Effective live/dead Stain Anonymous ReviewTo further characterize cell populations it is best to initially gate on live cells. in this experiment I used PI as my live/dead stain.

Propidium Iodide Solution from BioLegend

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Propidium Iodide Solution from BioLegend

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