Miltenyi Biotec
CD205 (DEC205) Antibody, anti-mouse, PE-Vio® 615, REAfinity™
130-112-270
REA817
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of B cells and antibody-secreting cells (ASC) in health and disease, in this experiment, we are looking for markers of PC.
Flow Cytometry
Mouse WT spleen
Surface staining with CD205PE-Vio615 (1/100) , 20 min at 4C
Fc block (2.4G2 clone)
N/A
Flow cytometer
In this work, we were looking for surface markers that are differentially expressed by B cells (CD19+) and plasma cells (PC). We found that CD205 (as known as DEC205) is higher expressed by PC (CD138+) in comparison with B cells.
35464469
Works and is cheap.
It is not very bright.
Not bright, but useful and cheap.