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Date Published
Accessible Anti-Cyclin A Antibody (B-8) for Western Blot
(1)
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Santa Cruz Biotechnology, Inc.
June 27, 2022
Cyclin A is a crucial component of the cell-cycle machinery. This antibody was used to detect cyclin A protein levels in human cell lysates...
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Good Anti-HIV-1 Core Protein Antibody, Tricky but Effective Staining Protocol
(1)
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June 27, 2022
This antibody is essential in our laboratory, as we perform several experiments in which strategies to reduce HIV-1 infection are performed....
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Efficient Human Fc Block
(1)
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BD Biosciences
June 27, 2022
I use this reagent in all flow cytometry experiments to prevent non-specific Fc receptor antibody binding. This reagent effectively blocks ...
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Identifying Endothelial Cells Using BD's Rat Anti-Mouse CD31 (PECAM-1) Antibody
(1)
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BD Biosciences
June 27, 2022
We employ BD's rat anti-mouse CD31 antibody for the detection of endothelial cells via PECAM-1 in brain tissue sections taken from our ...
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Great DNA Polymerase for GC Rich PCR
(1)
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June 27, 2022
We are trying to clone GC-rich DNA fragments from genomic DNA in the mouse liver. This is a great DNA polymerase that works well in ...
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Effect of Chemoattractant on AKAP7 Expression
(1)
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Proteintech Group Inc
June 24, 2022
AKAP7 have been shown to regulate neuronal ion channels. AKAP7alpha is the first AKAP7 splice variant identified and it is a membrane bound ...
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Anti-IBA1 for Staining Microglia in Murine Brain Tissue
(1)
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June 24, 2022
Fujifilm's anti-IBA1 polyclonal antibody is one of the best reagents used in our lab for staining of murine brain tissue sections. We use it...
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Anti-GFAP for Staining Astrocytes in Murine Brain Tissue Sections
(1)
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BD Biosciences
June 24, 2022
We use BD's murine anti-GFAP cocktail to stain astrocytes in brain sections obtained from our mouse models of neurodegeneration. The ...
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Great Fluorescent Ca2+ Indicator
(1)
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Thermo Fisher Scientific
June 24, 2022
We are interested in studying a class of novel circulating peptides. Preliminary data indicate some peptides may have roles in modulating ...
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Excellent VLGUT2 Antibody for Glutamatergic Detection
(1)
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Synaptic Systems GmbH
June 24, 2022
We use this VGLUT2 antibody to stain glutamatergic terminals in murine brains for subsequent visualization by confocal microscopy.
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Time Course Effect of Chemoattractant on KCNT2 Expression
(1)
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Thermo Fisher Scientific
June 24, 2022
KCNT2 gene encodes a subunit of a voltage-gated potassium channel that is activated by an increase of cytoplasmic Na+ concentration. It ...
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Time Course Effect of Chemoattractant on Anoctamin-1 Expression in Arteries
(1)
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Cell Signaling Technology
June 24, 2022
Anoctamin-1 also called TMEM16A. It is a voltage-gated calcium-activated anion channel, which function both as a chloride channel and a ...
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Robust Effect of Chemoattractant on ORAI1 Expression
(1)
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Thermo Fisher Scientific
June 24, 2022
Orai1 is a calcium selective ion channel that is encoded by the ORAI1 gene. Orai channels play crucial role in the activation of T-...
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Phospho-Rb (Ser807/811) Antibody for Western Blot
(1)
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Cell Signaling Technology
June 24, 2022
This antibody was used to detect phospho-Rb (retinoblastoma protein) expression from human cell lysates in western blot analysis. Rb ...
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Excellent 5-Laser, 19-Channel Flow Cytometer
(1)
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June 23, 2022
Our centre has one of these cytometers in our core facility. It is a great cytometer that is easy and convenient to use. It has the ability ...
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Great NRF2 Antibody
(1)
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BioLegend
June 23, 2022
I used a lot of anti-NRF2 antibodies and this was the best! It is anti-rat, which is a rare secondary species, so I can usually use it with ...
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Excellent MHC Class I Antibody
(1)
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BioLegend
June 23, 2022
I used this antibody to test MHC class I expression in NK cells after expansion in vitro. The antibody works great with very little ...
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Great Anti-NeuN Antibody to Stain Neurons in Murine Brain Sections
(1)
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MilliporeSigma
June 23, 2022
We utilize Anti-NeuN clone A60 to stain for neurons in brain tissue from our murine models of neurodegeneration. The antibody provides clear...
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Time Course Effect of Chemoattractant on KCNMB1 Expression
(1)
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Abcam
June 23, 2022
Large-conductance calcium and voltage-dependent potassium BK channel KCNMB1 encodes the ß1 subunit present in the vascular smooth muscle. ...
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Great Lymphoprep Density Gradient Medium
(1)
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June 23, 2022
I use this reagent for isolating mononuclear cells from fresh blood. It works great and requires no pre-mixing or diluting prior to use. It ...
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Not Effective for Western Blot
(1)
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Abcam
June 23, 2022
I performed Western blot on a control sample of human cortical organoids and a NGN2 knockout line, but the NGN2 antibody did not work. I do ...
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Actin Antibody Works Great
(1)
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MilliporeSigma
June 23, 2022
I used this antibody for Western blot on protein extracted from human cortical organoids and it worked perfectly! The bands came up at the ...
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My Favorite Western Transfer System
(1)
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Bio-Rad
June 22, 2022
Me and everyone in my lab does routine western blots to measure the quantity of proteins in my cells. I usually either measure existing ...
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Array is Very Easy To Use and Provides A LOT of Information
(1)
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RayBiotech
June 22, 2022
I wanted to compare the differences in cytokines between two differentially mutated cancer cell lines and decided to use this instead of ...
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Excellent Antibody for Phospho-Y1068 EGFR Signal
(1)
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Cell Signaling Technology
June 22, 2022
We generated a knockout of a member of the JAK family of proteins and wanted to examine whether there were changes in EGFR signaling. We ...
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Good Western Blot Antibody for Cas9 Protein
(1)
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Cell Signaling Technology
June 22, 2022
We transduced a Cas9 plasmid in multiple cancer cell lines. We used this antibody to validate the expression of Cas9 in these cell lines ...
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Efficient qRT-PCR Using Invitrogen's SuperScript III First-Strand RTase
(1)
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June 22, 2022
Our laboratory employs Invitrogen's SS III RTase/system for all of our qRT-PCR work. The reagent is reliable and provides high quality, ...
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Bright CFSE Stain
(1)
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MilliporeSigma
June 22, 2022
I use this reagent for staining cells in a cytotoxicity assay. Cells are easily stained with this dye non-discriminately, and it gives a ...
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Mounting Tissue Samples with ProLong Gold Antifade Reagent
(1)
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June 22, 2022
Before downstream imaging, we use ProLong Gold antifade mountant on our murine brain tissue samples. The product allows for the ...
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Good BIM Antibody for Upper Isoform Quantification
(1)
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Cell Signaling Technology
June 22, 2022
We knocked out an upstream JAK protein (JAK KO) and wanted to examine if there were changes in BIM expression in JAK KO cells. We examined ...
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Great Human CD70 Recombinant Protein
(1)
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Thermo Fisher Scientific
June 22, 2022
I used this protein to detect CAR expression in some anti-CD70 CAR-NK cells. The protein has an Fc tag which can be detected using an anti-...
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Stoelting's Y Maze is Great for Assessing Spatial Recognition Memory
(1)
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June 15, 2022
We used the Y maze for mice from Stoelting to assess and compare spatial recognition memory in multiple murine cohorts with memory deficits ...
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Great IgD Antibody with Strong Staining
(1)
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Thermo Fisher Scientific
June 15, 2022
I use this antibody frequently to stain B cells from mouse lymph nodes for flow cytometry. It works great and the staining is strong.
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Good Polyclonal Prox-1 Antibody
(1)
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BioLegend
June 15, 2022
I used this antibody to detect lymphatics in mouse lung tissue. I couldn't use a LYVE-1 antibody for this application since all blood ...
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Good Phospho-STAT3 Antibody for Protein Quantification
(1)
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Cell Signaling Technology
June 15, 2022
We knocked out an upstream JAK protein in the Cal27 cell line and wanted to examine downstream STAT3 signaling. We blotted JAK KO (KO) and ...
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Fixable Viability Dye
(1)
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June 15, 2022
I use this dye in flow cytometry experiments to assess viability in samples that will be fixed later on. It works well to stain dead cells ...
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Isolation Cubicle Used in Murine Fear Conditioning
(1)
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Coulbourn Instruments
June 15, 2022
We use Colbourn's isolation cubicle to set up and house our fear conditioning experiments on murine models. The isolation cubicle is the ...
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Good Antibody for Cathepsin B
(1)
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Proteintech Group Inc
June 15, 2022
We used this antibody to test the levels of Cathepsin B protein in the endosomal compartments and cytoplasmic fractions of senescent versus ...
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Great Human CD70 Antibody
(1)
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BioLegend
June 15, 2022
I needed this antibody to test CD70 expression on expanded NK cells. The antibody worked well right away with good staining and barely any ...
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Human IgG Fc Antibody
(1)
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BioLegend
June 15, 2022
I use this antibody as a secondary antibody to detect binding of an Fc-tagged recombinant protein to my CAR-NK cells. The antibody yields ...
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Excellent CD138 Antibody With Good Staining
(1)
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BD Biosciences
June 15, 2022
I frequently use this antibody from BD Biosciences to stain immune cells from mouse lymph nodes for flow cytometry. It works great and the ...
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Elevated Zero Maze to Characterize Mouse Anxiety-Like Behavior
(1)
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June 14, 2022
We used Stotelting's elevated zero maze to character the anxiety-like behavior in murine cohorts. The zero maze is easy to set up and use, ...
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Excellent CD80 Antibody with Strong Staining
(1)
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BD Biosciences
June 14, 2022
I use this antibody from BD Biosciences frequently to stain immune cells from mouse lymph nodes for flow cytometry. It works great and the ...
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Strong Staining with PE-Cy™7 Antibody
(1)
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BD Biosciences
June 14, 2022
I use this antibody frequently to stain T and B cells from mouse lymph nodes for flow cytometry. This antibody works great, reliable and ...
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Good TFAM Antibody for Western Blot
(1)
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Cell Signaling Technology
June 14, 2022
Cells were seeded and left overnight at 37 °C and 5% CO2. The day after were stimulated for 24 hours with 0.5% FBS. I harvested with lysis ...
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Fast and Large-Scale Plasmid DNA Prep Without Centrifugation
(1)
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QIAGEN - Sample to Insight
June 14, 2022
One of our research is focused on B7-H3 (or CD276), a molecule of the B7 family, which may be a promising target for cancer treatment as is ...
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Great GL7 Antibody with Bright Staining
(1)
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BioLegend
June 14, 2022
I use this antibody frequently to stain immune cells from mouse lymph nodes for flow cytometry. It works great and the staining is strong. ...
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Bright CD95 Antibody with Good Staining
(1)
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BioLegend
June 14, 2022
I use this antibody frequently to stain immune cells from mouse lymph nodes for flow cytometry. It works great and has excellent signal, ...
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Great pAMPKa Antibody for Western Blot
(1)
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Cell Signaling Technology
June 14, 2022
I seed the cells and I wait 24 hours leaving them at 37 °C, 5% CO2. I stimulated the cells for 24 hours and I harvested with lysis buffer ...
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Great Human CD3 Antibody
(1)
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BioLegend
June 14, 2022
I use this antibody in flow cytometry experiments to detect T cells. The antibody gives a strong signal with little to no background and is ...
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Camera
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Western Blot
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Cell Culture
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qPCR
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Concentration
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Mutagenesis
FISH
CRISPR
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In Vivo Imaging
EMSA
Cell Fractionation
Immunoassay
In Vitro Transcription
ChIP-Seq
Northern Blot
3D Imaging
RNAi
ELISPOT
PCR Cloning
Particle Analysis
Southern Blot
Protein Modification
DNA Analysis
Expression
Cell-Based Assay
Gene Editing
In Vivo Testing
Protein Labeling
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Human
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Arabidopsis
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Virus
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Drosophila
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Tissue
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Adipose
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In Vivo
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Peripheral Blood
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Cell Type
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Adipocyte
Astrocyte
B Cell
CD8+ T Cell
Competent Cells
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Induced Pluripotent Stem Cell
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Macrophage
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Microvascular Endothelial Cell
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PBMC
Platelets
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Thymocytes
Tumor Cells
Cell Line
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HeLa
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U2OS
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HL60 (HL-60)
A375 (A-375)
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MCF-7
A431
MDA-MB-231
A549
N2a
Daudi
HCT-116
Raji
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