Cell Signaling
Cyclin D1 Antibody
2922
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I seeded MCF-7 (invasive ductal carcinoma) with complete medium 10% FBS; then I stimulated the cells for 24 hours in starvation medium (0.5% FBS). I detected the protein (Cyclin D1) with western blot. The membrane was incubate overnight at 4 °C
Western Blot
MCF-7
1:1000 overnight at 4 °C
BSA 5% in 0.1% TBST
Rabbit RT for 2 hours
Didn't use.
ChemiDOC
I seeded MCF-7 (invasive ductal carcinoma) with complete medium 10% FBS; then I stimulated the cells for 24 hours in starvation medium (0.5% FBS). I detected the protein (Cyclin D1) with western blot. The membrane was incubated overnight at 4°C.
Accessible and high quality.
Nothing bad.
Antibody showed great signal.