Miltenyi Biotec
CD268 Antibody, anti-mouse, PE-Vio® 770, REAfinity™
130-123-436
REA1217
Info:Supplier Page View Product Specs
Browse Similar Products: CD268 Antibodies
Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of B cells and antibody-secreting cells (ASC) in health and disease, in this particular experiment, we are titrating an anti-CD268 expressed on B cells.
Flow Cytometry
Mouse spleen
Surface staining with CD268 PE-Vio 770 (1/100), 20 min at 4C
Fc block (2.4G2 clone)
N/A
Flow cytometer A5 Symphony BD
Splenocytes were gated for live lymphocytes. CD19 is a B cell marker was used to gate B cells. Within B cells several dilutions of CD268 were tested. 1/100 dilution is the ideal dilution.
It works well.
It is a good and cheap option to stain mouse CD268.