Cell Signaling
Caspase-8 (D35G2) Rabbit mAb
4790S
N/A
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I seeded the epithelial cells for 24 hours. I starved the cells for 4 hours (0% FBS) and I induced for 24 hours with PDFG and treated simultaneously with eiF4E siRNA in 2%FBS. I didn't get a clear band
Western Blot
Epithelial cells
1:1000 overnight at 4 °C
BSA 5% in 0.1% TBST
Rabbit RT for 2 hours
Didn't use.
ChemiDOC
I seeded the epithelial cells for 24 hours. I starved the cells for 4 hours (0% FBS) and I induced for 24 hours with PDFG and treated simultaneously with eiF4E siRNA in 2%FBS. I didn't get a clear band.
Not clear.
Band not clear.
Not really recommended.