Great APC Anti Human CD86

July 15, 2022

BlueskyReddit
Houston Methodist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

APC anti human CD86

Catalog Number:

374208

Clone Number:

BU63

Info:Supplier Page View Product Specs

Browse Similar Products: CD86 Antibodies

This allows me to evaluate the polarization status and activation of immune cells. Along with CD80, CD86 provides costimulatory signals necessary for immune cell activation and survival. Depending on the ligand-bound, CD86 can signal self-regulation and cell-cell association or attenuation of regulation and cell-cell disassociation.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Cells and tissue

Primary Incubation

4ul/ 500.000 - 1,000.000 cells

Blocking Agent

BSA 1% in PBS or goat serum 1% in pBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Cytofluorimeter

Results Summary

Evaluation of immune cell activation / inflammatory status under specific treatment conditions. you need sometime to use more ul because the signal is a bit low.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very sensitive and specific signal for flow cytometry.

The Bad

Depending on the quality of sample, sometimes you need to use higher Ab concentration to obtain a good signal.

The Bottom Line

Overall the antibody is good, sometime you need to use higher Ab concentration to obtain a good signal.

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