Beckman Coulter
CD45-PC7 Conjugated Antibody, J33, 1 mL, ASR
IM3548U
J33
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Browse Similar Products: CD45 Flow Cytometry Antibodies
It is almost universal that labs studying human WBCs need a common leukocyte marker to distinguish the WBC population from debris, RBCs and platelets. We use CD45 in all our panels and therefore need it in different conjugates. Here, we selected CD45 PC7 and show that it works very well.
Flow Cytometry
Human blood
10 minutes at RT
Bovine serum (1%)
N/A
FL-5 channel (Navios flow cytometer)
Results show very good separation between the WBCs (lymphocytes in red, monocytes in green, and granulocytes in blue) compared to non-leukocytes (i.e. debris, RBC and platelets in grey). This allows us to gate on the WBCs and clean up the data significantly, especially in less than optimal specimens.
Easy to use, good signal.
More expensive than other conjugates.
This is a good antibody that served its purpose in our lab's testing.