Great EGF Receptor Antibody for Western Blot

July 12, 2022

NanoEngineering
UC San Diego NanoEngineering Department
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling

Product Name:

EGF Receptor Antibody

Catalog Number:

2232

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: EGF Antibodies

I used A431 as cell type. The cells were seeded with starvation medium and treated for 24 hours. I used rabbit GE as secondary antibody. The membrane was incubated at 4°C overnight first, then with secondary antibody at RT for 2 hours.

Experimental Design and Results Summary

Applications

Western blot

Sample

A431

Primary Incubation

1:1000 overnight at 4 °C

Blocking Agent

BSA 5% in 0.1% TBST

Secondary Incubation

Rabbit RT for 2 hours

Tertiary Incubation

Didn't use.

Detection

ChemiDOC

Results Summary

I used A431 as cell type. The cells were seeded with starvation medium and treated for 24 hours. I used rabbit GE as secondary antibody. The membrane was incubated at 4°C overnight first, then with secondary antibody at RT for 2 hours.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Band is clear.

The Bad

N/A

The Bottom Line

I recommend to use high voltage due to high molecular weight.

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