Good MHCII Antibody for Flow Cytometry

July 08, 2022

MCB
UC Berkeley
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher

Product Name:

Anti-mouse MHCII (I-A/I-E) eF450

Catalog Number:

48-5321-80

Clone Number:

M5/114.15.2

I use this MHCII antibody frequently to stain immune cells from mouse lymph nodes for flow cytometry. It works great and the staining is strong.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse lymph nodes

Primary Incubation

1:200, 30min, 4C

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

I use this antibody frequently to stain immune cells from mouse lymph nodes for flow cytometry. It works great and the staining is strong.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Reliable and good signal.

The Bad

None

The Bottom Line

Antibody has great signal, highly recommend.

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