Reliable Viability Dye

UMass
Pathology
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Beckman Coulter

Product Name:

7-AAD Viability Dye, 150 Tests, CE, IVD

Catalog Number:

A07704

Viability assessment is important especially in older specimens and ones that have been mechanically disaggregated e.g., lymph nodes and other tissues. We needed a good viability dye that would allow us to distinguish dead from viable cells. We chose this dye for that purpose.

Experimental Design and Results Summary

Application

Flow cytometry

Starting Material

Single cell suspension

Tips

Must be run within an hour of adding the dye.

Results Summary

The results show decent separation of dead (7AAD+) events shown in red compared to the viable cells that do not allow 7AAD to enter the cell (shown in green).

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Easy to use, simple addition.

The Bad

Cannot use with fixed or permeabilized cells.

The Bottom Line

This is a simple viability dye that will be very useful to labs helping them distinguish dead from viable cells.

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