DAPI Nuclear Stain

McMaster University
MIRC
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

DAPI (4',6-Diamidino-2-Phenylindole, Dilactate)

Catalog Number:

422801

I use this reagent for routine staining of nuclei in immunofluorescence. It works great in most samples and gives a bright clear signal.

Experimental Design and Results Summary

Application

Immunofluorescence

Starting Material

Rat lung section

Tips

Reagent needs to be diluted for use.

Results Summary

The stain works well to identify nuclei in the section. Just dilute the reagent as recommended then put a drop on the section, wash, then mount. Works great every time.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Easy to use.

The Bad

Needs to be diluted.

The Bottom Line

You really don't need much to get good staining, so it will last a long time. It's easy to use and gives good results.

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