Thermo Fisher Scientific
SuperSignal West Pico Chemiluminescent Substrate
34577 (previously 34087)
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Cells at 80% confluency in 35-mm cell culture dishes are lysed in ice-cold 100 μl RIPA buffer containing 1 μl protease inhibitor cocktail. Supernatant from cell lysates is used for gel electrophoresis followed by western blot. Nitrocellulose or PVDF membrane containing protein bands are blocked and incubated with primary and secondary antibodies. The protein bands are then detected using SuperSignal West Pico Chemiluminescent Substrate in ChemiDoc MP Imaging System.
Detect protein bands in nitrocellulose or PVDF membrane in western blot.
Cell lysates, western blot, antibodies.
Incubate the membrane with the substrate for few minutes before detection.
Protein bands bound to HRP-conjugated secondary antibodies react with the SuperSignal West Pico PLUS substrate, which are visualized using ChemiDoc MP Imaging System.
N/A
Highly sensitive to detect picogram protein in blots.
None
SuperSignal West Pico is suitable to detect protein bands in the nitrocellulose/PVDF membrane.