Great Results With Western Blot GFP

July 12, 2022

NanoEngineering
UC San Diego NanoEngineering Department
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling

Product Name:

GFP Antibody

Catalog Number:

2555s

Clone Number:

N/A

Info:Supplier Page View Product Specs

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This antibody has been working well for western blots, I have never had a problem with it. I use a 1:1000 dilution in BSA. Cell type: HEK293

Experimental Design and Results Summary

Applications

Western blot

Sample

HEK293

Primary Incubation

1:1000 overnight at 4 °C

Blocking Agent

BSA 5% in 0.1% TBST

Secondary Incubation

Rabbit RT for 2 hours

Tertiary Incubation

Didn't use.

Detection

ChemiDOC

Results Summary

This antibody has been working well for western blots, I have never had a problem with it. I use a 1:1000 dilution in BSA. Cell type: HEK293

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Accessible and high quality.

The Bad

Nothing bad.

The Bottom Line

Antibody is reliable with great signal.

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