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Date Published
Spike Protein Effectively Stimulates Immune Cells
(1)
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R&D Systems, a Bio-Techne Brand
July 06, 2022
Here, we stimulated human macrophages with this protein to measure downstream inflammatory markers. The protein was pure with no evidence of...
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Fast and Efficient Trans-Blot Turbo™ Transfer System
(1)
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Bio-Rad
July 06, 2022
The proteins from HEK293 cell lysate were resolved in NuPAGE 4%–12% Bis-Tris gel and transferred to 0.2-µm PVDF membrane for 7 minutes with ...
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Opti-MEM is a Great Medium for Transfecting Plasmid DNA into Cells
(1)
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July 06, 2022
0.5 µg of ER-localized mEmerald-ER3 DNA and 0.75 µl fugene HD were diluted in 200 µl Optimem and incubated for 15 min. The complex was added...
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Suitable Magnetic Beads for Immunoprecipitation of c-Myc-Tagged Proteins
(1)
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Thermo Fisher Scientific
July 06, 2022
200 ug/ml lysate from HEK293 cells expressing recombinant c-Myc-tagged proteins is incubated with 50 ug Pierce Anti-c-Myc magnetic beads in ...
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Sartorius Mechanical Pipettes are Ideal for Routine Lab Use
(1)
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Sartorius
July 06, 2022
We routinely use 0.5-10 ul, 10-100 ul and 100-1000 ul pipettes for pipetting a small amount of solutions in western blot, qPCR, cloning, ...
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Excellent 12 Well Gel for Western Blot
(1)
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Bio-Rad
July 06, 2022
I used these gels several times for my western blot, and I have always gotten great results. I have never had a bad experience, bands are ...
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Cell Culture Testing of S2 Sub Unit
(1)
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R&D Systems, a Bio-Techne Brand
July 06, 2022
Here we tested the S2 sub unit of the spike protein for immunogenicity against human macrophages. The protein was treated in cell culture ...
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Great Cell Permeant Stain for Flow Cytometry
(1)
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July 06, 2022
Nitric oxide is a member of the reactive nitrogen species important for a variety of functions including antimicrobial actions and cellular ...
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Reliable Nickel Resin for His Tag-Based Protein Isolation
(1)
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July 05, 2022
We are interested in studying a new secreted protein in mice. We use this resin to purify this His-tagged protein expressed in 293T cells. ...
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Great Mini Shaker
(1)
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July 05, 2022
Our lab does some assays that require a plate to be shaking at a specific speed and temperature, with the option of adding humidity (using ...
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Great Mini Centrifuge for Different Sized Tubes
(1)
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July 05, 2022
We spin down a lot of cells and reagents and wanted a mini centrifuge that can fit different-sized tubes. Great mini centrifuge, takes up a ...
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Digidata 1440A is Ideal for Whole-Cell Electrophysiology
(1)
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Molecular Devices
July 05, 2022
Digidata 1440A is routinely used in our lab together with axopatch 200B amplifier for whole-cell patch-clamp recording of ionic currents and...
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Herasafe™ 2030i is Suitable for Sterile Handling of Cell Culture
(1)
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Thermo Fisher Scientific
July 05, 2022
We routinely use Herasafe™ 2030i for sterile handling of cell culture. Under laminar flow, we split and seed the cell lines in a sterile ...
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Reliable Water Bath
(1)
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July 05, 2022
We have a lot of frozen samples, and this bath works well with both water and beads to thaw them evenly and quickly. Easy to use, consistent...
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SZX7 is Suitable to Visualize Tissue Samples
(1)
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Evident
July 05, 2022
We use SZX7 Zoom Stereo Microscope to visualize mouse sinoatrial node-atrial tissue for recording extracellular field potential during ...
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Axopatch 200B is Ideal for Whole Cell Electrophysiology
(1)
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Molecular Devices
July 05, 2022
Axopatch 200B is routinely used in our lab for whole-cell patch-clamp recording of guinea pig cardiomyocytes. Voltage-clamp mode is used to ...
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Great Mouse Monocyte Isolation Kit
(1)
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July 05, 2022
We are interested in studying a new secreted protein from hepatocytes. Previous research suggests that this protein may have a function in ...
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Great Anti-VGLUT1 Monoclonal Antibody for Western Blot
(1)
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Thermo Fisher Scientific
June 30, 2022
We used this product to test the expression of the VGLUT1 protein. The antibody works well at the suggested dilution but gives some ...
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MaxQ™ 4000 is an Ideal Shaker & Incubator for Bacterial Culture
(1)
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Thermo Fisher Scientific
June 30, 2022
MaxQ™ 4000 Benchtop Orbital Shaker is routinely used for bacterial suspension culture in 1-2 L conical flask or 15 ml falcon tubes at 200 ...
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Microcentrifuge for Efficient Centrifugation of Lab Samples
(1)
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Thermo Fisher Scientific
June 30, 2022
This microcentrifuge accelerates quickly up to 21,000xg to centrifuge samples in 1.5 to 2.0 mL centrifuge tubes, and the temperature can be ...
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Great Dry Heating Block
(1)
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June 30, 2022
We do a lot of assays that require heat for enzymatic reactions and need a nice small dry block that will heat the samples high without ...
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Fast, Easy and Multipurpose Fluorometer
(1)
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June 30, 2022
We use this regularly in the lab to measure protein, RNA and DNA (almost daily). It is a small, compact machine that takes up very less ...
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Good Western Blot Antibody to Measure EGFR Phosphorylation Levels
(1)
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Cell Signaling Technology
June 30, 2022
We treated a HNSCC cell line in the presence or absence of the EGF ligand. We wanted to determine whether the ligand was able to activate ...
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Great Vortexer
(1)
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Scientific Industries, Inc.
June 30, 2022
As a wet lab, we have a lot of samples to mix for various assays and need an easy to use and quiet vortexer that takes a small amount of ...
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Easy to Use Nanodrop
(1)
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June 30, 2022
We study immune cells extracted from human tissues in order to study their function. In our studies, we extract DNA for analysis and need a ...
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Good Antibody to Detect MAP2-Neuronal Marker
(1)
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Abcam
June 29, 2022
We used this antibody to detect MAP2 expression in mammalian cells. The antibody works well at the suggested dilution with some cross ...
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BHQ Depletes ER Ca2+ Stores in HEK Cells
(1)
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Tocris Bioscience, a Bio-Techne Brand
June 29, 2022
HEK293 cells were incubated with intracellular Ca2+ sensitive dye, Fura-2AM in normal tyrode solution for 30 min, washed and kept aside for ...
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Reasonably Good Antibody to Detect RFP-Tag
(1)
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Rockland Immunochemicals, Inc.
June 29, 2022
We have been using this antibody to detect RFP tags in recombinant proteins. The antibody is specific and works well at the suggested ...
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Good Western Blot Antibody to Measure ERK1/2 Phosphorylation Levels
(1)
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Cell Signaling Technology
June 29, 2022
We treated a HNSCC cell line in the presence or absence of the EGF ligand. We wanted to determine whether the ligand was able to activate ...
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Excellent Antibody to Detect Caspase-3
(1)
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MilliporeSigma
June 29, 2022
This antibody works really well and it's a good antibody to assess cell death by detecting Caspase-3 levels. Very good antibody to assess ...
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2-APB Reduces Store-Operated Ca2+ Entry in HEK293 Cells
(1)
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Tocris Bioscience, a Bio-Techne Brand
June 29, 2022
HEK293 cells were incubated with intracellular Ca2+ sensitive dye, Fura-2AM in normal tyrode solution for 40 min, washed and kept aside for ...
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PowerPac™ Basic Power Supply is Great for Gel Electrophoresis & Blotting
(1)
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Bio-Rad
June 29, 2022
The power supply is routinely used for gel electrophoresis at constant voltage 200V for 30-60 minutes, followed by transfer to PVDF or ...
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Great T Cell Activator
(1)
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June 29, 2022
Our lab studies the function of immune cells in human tissues, and we need a good activator of T cells specifically for downstream studies, ...
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Cyclopiazonic Acid Depletes ER Ca2+ Stores in HEK Cells Leading to Store-Operated Ca2+ Entry
(1)
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Tocris Bioscience, a Bio-Techne Brand
June 29, 2022
HEK293 cells were incubated with intracellular Ca2+ sensitive dye, Fura-2AM in normal tyrode solution for 30 min, washed and kept aside for ...
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Active Place Avoidance Apparatus to Assess Murine Spatial Navigation & Memory
(1)
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June 28, 2022
We utilized MazeEnginners APA apparatus for mice to test spatial learning and spatial memory in multiple mouse models affected by ...
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Robust Bcl-w (31H4) mAb for Western Blot
(1)
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Cell Signaling Technology
June 28, 2022
This antibody was used to detect BCL-W levels from human cell lysates (25 ug/mL protein loading) through western blot analysis.
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Excellent Antibody for Flow Cytometry of Tissue Resident Memory Cells in Human Tissues
(1)
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BioLegend
June 28, 2022
We use this antibody to study the phenotype of TRM NK cells in several tissues. It stains better than desired.
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Staining for Microglia in Mouse Brain Sections
(1)
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Synaptic Systems GmbH
June 28, 2022
Our lab utilizes Synaptic System's anti-IBA 1 antibody to stain for microglia in brain sections from our murine cohorts of neurodegeneration...
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Efficient ß-Tubulin Antibody for Western Blot
(1)
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Cell Signaling Technology
June 28, 2022
This antibody was used as a loading control for western blot analysis. Cell lysates (25 ug/mL protein) were used.
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Efficient Transfection of Plasmid DNA into HEK293 Cells
(1)
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Promega
June 28, 2022
1 µg of STIM1-CFP plasmid and 2 µl transfast reagent were diluted in 200 µl Optimem and incubated for 15 min. The complex was added to the ...
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Millipore's Anti-GAD67 for Staining of GABAergic Synaptic Terminals
(1)
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MilliporeSigma
June 28, 2022
In brain tissue sections taken from our murine cohorts of neurodegeneration, we use Millipore's Anti-GAD67 antibody for staining GABAergic ...
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GAPDH (D16H11) XP Rabbit mAb for Western Blot Analysis
(1)
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Cell Signaling Technology
June 28, 2022
This antibody was used as a loading control for western blot analysis. Human cell lysates were used (25 ug/mL) in the experiment.
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Good Antibody to Detect Phospho-FAK
(1)
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MilliporeSigma
June 28, 2022
We used this antibody to detect the phosphorylation levels of FAK protein in the HeLa cell line by western blot analysis. The antibody works...
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Staining Myelin in Murine Brain Tissue Sections Using Invitrogen's FluoroMyelin Red
(1)
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June 27, 2022
To stain for myelin in the white matter brain tissue sections taken from our murine cohorts of neurodegeneration, we use Invitrogen's ...
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Effect of Chemoattractant on Thioredoxin-1 Expression
(1)
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Cell Signaling Technology
June 27, 2022
Trx1is a 12-kD multifunctional antioxidant protein with a redox-active disulfide/dithiol within the conserved active site Cys32–Gly–Pro–...
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Great ALT ELISA Kit
(1)
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MyBioSource.com
June 27, 2022
We are exploring the potential endocrine functions of a class of novel circulating peptides. Some peptides may have impact liver health. We ...
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Easy to Use ELISA Kit, Suitable for Multiple Cells
(1)
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R&D Systems, a Bio-Techne Brand
June 27, 2022
This kit was used to determine the concentration of this protein-of-interest in multiple cancer cell lines and in different conditions. Just...
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Bcl-2 (124) Mouse mAb for Western Blot
(1)
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Cell Signaling Technology
June 27, 2022
The BCL-2 family proteins have multiple functions; among these, BCL-2 is an apoptosis regulator. This antibody was used to detect BCL-2 ...
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Sensitive P16 INK4A Rabbit mAb for Western Blot
(1)
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Cell Signaling Technology
June 27, 2022
p16 is a tumor suppressor protein that plays an important role in regulating the cell cycle. This antibody was used to detect p16 levels in ...
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Robust Bcl-xL (54H6) mAb for Western Blot
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Cell Signaling Technology
June 27, 2022
The BCL-2 family proteins have multiple functions; among these, BCL-XL is known to inhibit apoptosis. This antibody was used to detect BCL-...
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