R&D Systems
PARP/Apoptosis Universal Colorimetric Assay Kit
4677-096-K
N/A
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In our lab we study PARP-1 that is a nuclear protein and mediates Poly-ADP-ribosylation o nuclear proteins (as it is, and histones for example), a process that occurs in response to DNA damage. Inhibiting PARP has shown to promote chemo/radiosensitization of tumors in the conventional therapy of malignant tumors. Furthermore, PARP1 inhibitors can be used as independent, effective drugs against tumors with broken DNA repair mechanisms.Our aim was doing a vitro screening of a candidate PARP-1 inhibitor in order to determine its IC50 value. As we know PARP-1 release a biotinylated poly (ADP-ribose) that attach the 96-wells surface coated with histones. Detections of PARP-1 was made through the absorbance (at 450nm) of the colorimetric HRP substrate of Strep-HRP (a biotin binding protein). We found a reliable value of IC50. We will proceed testing other inhibitors with this kit!
ELISA
Inhibitor of PARP-1
Absorbance at 450nm
In our lab we study PARP-1 that is a nuclear protein and mediates Poly-ADP-ribosylation o nuclear proteins (as it is, and histones for example), a process that occurs in response to DNA damage. Our aim was doing a vitro screening of a candidate PARP-1 inhibitor in order to determine its IC50 value. Inhibiting PARP has shown to promote chemo/radiosensitization of tumors but can be used as independent, effective drugs against tumors with broken DNA repair mechanisms.As we know PARP-1 release a biotinylated poly (ADP-ribose) that attach the 96-wells surface coated with histones. Detections of PARP-1 was made through the absorbance (at 450nm) of the colorimetric HRP substrate of Strep-HRP (a biotin binding protein). We found a reliable value of IC50. We will proceed testing other inhibitors with this kit!
26483957
Photo attached (Histone-Coated Clear Strip Wells, 96-well _ Cat. No. 4677-096-P)
Reliable and precise results (IC50 values), easy to use.
Confusing protocol (units of measure used to indicate the same substance change).
Clear and reliable results, easy to follow protocol.