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Date Published
TIM-4 Expression of LPS Activated Peritoneal Macrophages
(1)
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BioLegend
April 11, 2014
To detect the TIM-4 expression on LPS activated peritoneal macrophages.
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StepOnePlus is a Step Above the Rest
(1)
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Thermo Fisher Scientific
April 11, 2014
Research in our lab focuses on how temperament or personality relates to physiology and health. The larger goal is to determine how and why ...
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Detection of Low Abundant Proteins by Chemiluminescent Western Blotting
(1)
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Advansta
April 08, 2014
We are interested in understanding the regulation of protein stability of those important low abundant proteins with short half-life in ...
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Anti-Brg-1: Good for Immunofluorescence
(1)
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Santa Cruz Biotechnology, Inc.
April 08, 2014
The purpose of this experiment was to identify an antibody that was suitable for detection of BRG-1 in human cells by indirect ...
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Strong Signal: Anti-BMI1 Immunofluorescence
(1)
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MilliporeSigma
April 07, 2014
The purpose of this experiment was to identify an antibody that was suitable for detection of BMI1 in human cells by indirect ...
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Love This Machine!
(1)
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Thermo Fisher Scientific
April 04, 2014
We typically perform 20-40 gene expression experiments each week. This machine is fantastic in that you can create templates, drop in your ...
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Good PCR Machine for a Low Price
(1)
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Corning Life Sciences
April 03, 2014
We bought this thermocycler to run 24/7 and it does the job , its not fancy with touch screens / graphics!! Does the job and that's the best...
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Strong Signal: Anti-BRCA1 Immunofluorescence
(1)
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Santa Cruz Biotechnology, Inc.
April 02, 2014
The purpose of this experiment was to identify an antibody that was suitable for detection of BRCA1 in human cells by indirect ...
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The Standard for Comparing All Other PCR Instruments
(1)
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Roche
April 02, 2014
Easy enough to train undergrads on, but powerful enough for a senior research scientist, the Roche LightCycler 480 is the gold standard I ...
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Very Good: Anti-ATRX, Immunofluorescence
(1)
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Santa Cruz Biotechnology, Inc.
March 31, 2014
The purpose of this experiment was to identify an antibody that was suitable for detection of ATRX in human cells by indirect ...
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Strong Signal: Anti-Acetyl-Lysine Immunofluorescence
(1)
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MilliporeSigma
March 28, 2014
The purpose of this experiment was to identify an antibody that could detect all acetyl-Lysine by immunofluorescence. Lysine acetylation is ...
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TCR Detection by Anti-TCRa/b
(1)
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BioLegend
March 26, 2014
My research focuses on expressing T cell receptors using lentiviral vectors. I use Jurkat cells to test the vectors for TCR expression. I ...
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PEI - An Inexpensive Transfection Reagent
(1)
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March 25, 2014
My research involves making lentiviral vectors pseudotyped with vesicular stomatitis virus envelop glycoprotein (VSV-G). In this protocol, I...
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The Best PCR Purification Kit of All Times
(1)
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March 24, 2014
As a TCR-immunotherapy project, a part of my research includes cloning various inserts (T cell receptors) into lentiviral vectors as well as...
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Antibody for HA Tagging a Protein
(1)
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March 21, 2014
Hemagglutinin (HA) epitope tag, or HA tag, is one of the most usual ways to mark and detect a protein in vivo. Good antibody for HA tag is ...
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Good Results: Anti-SP3 and Anti-KLF6
(1)
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BioLegend
March 20, 2014
Purified anti-SP3 and purified anti-KLF6 antibodies give good results.
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Antibody for Ubiquitination Analysis
(1)
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MilliporeSigma
March 20, 2014
Ubiquitination is the most important way to regulate proteins in cells. Our lab has demonstrated that FbxL5 can direct SNA1 to degradation ...
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V5 Tag Detection
(1)
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Thermo Fisher Scientific
March 19, 2014
Protein tagging is a very usual method to detect proteins with no available good commercial antibodies. V5 epitope tag is derived from a ...
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Easy and Fast Estimation of Glucose Uptake
(1)
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Cayman Chemical
March 18, 2014
Cayman's Glucose Uptake Cell-based Kit allows simple, fast and accurate estimate of uptake of a fluorescent glucose analog by cells. Results...
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Excellent Anti HSA (CD24) Antibody With Great Fluorescence
(1)
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BioLegend
March 17, 2014
Anti HSA antibodies have an annoying feature that when they bind the negative control jumps above unstained (this is not overcome by adding ...
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Ubiquitination in Nuclei by FBXL5
(1)
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Santa Cruz Biotechnology, Inc.
March 14, 2014
SNA1 is the main effectors of EMT due to its gene repression activity. Stabilization and subcellular localization of the protein plays a key...
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Cell Cycle Analysis. CDK1 Detection by WB
(1)
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March 13, 2014
We focus our research in EMT induced by SNA1 transcription factor. One of the poorly understood mechanisms is the effects on cell cycle ...
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CD14: Good Signal, but Relatively High Background!
(1)
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BioLegend
March 12, 2014
CD14 is the LPS receptor, expressed at high levels on monocytes. When staining ficoll-paque isolated human PBMC, the monocytic gate is not ...
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Excellent Antibody for T-Cell Activation
(1)
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March 11, 2014
My research focuses on T cell based immunotherapy. For this, I regularly activate and expand primary T cells from PBMCs. Anti-CD3 antibody ...
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Clathrin Heavy Chain Antibody
(1)
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Cell Signaling Technology
March 10, 2014
A clean antibody to detect Clathrin heavy chain. High specificity but sensitivity is okay, load 30-40 ug total protein to get a strong ...
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CD169 (Siglec-1) Antibody: Works Out of the Box
(1)
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BioLegend
March 07, 2014
The aim of the research is to study lymph node myeloid cells in cancer. In my experiment, I tested if a cytokine associated with tumor ...
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CD11b Antibody: Brilliant!
(1)
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BioLegend
March 06, 2014
The aim of the research is to study splenic hemopoietic progenitor cells in cancer. In my experiment, I tested if a cytokine associated with...
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CD169 Antibody: Reliable Reagent
(1)
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Bio-Rad
March 05, 2014
The aim of the research is to study lymph node myeloid cells in cancer. In my experiment, I tested if a cytokine associated with tumor ...
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CD40 Antibody: Excellent Product
(1)
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BioLegend
March 04, 2014
The product used to determine macrophage activation following TLR stimulation.
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CD3, CD4, CD8 Antibodies
(1)
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BioLegend
March 03, 2014
These antibodies from BioLegend work extremely well. I used these to phenotype in vitro expanded primary T cells. These antibodies give me ...
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Reliable CD103 Antibody
(1)
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BioLegend
March 03, 2014
The aim of the research is to study splenic hemopoietic progenitor cells in cancer. In my experiment, I tested if a cytokine associated with...
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Maximizing Double Stranded RNA Synthesis
(1)
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Thermo Fisher Scientific
February 28, 2014
In vitro transcription of RNA transcripts has become an important laboratory technique for many cell and molecular biology experiments. The ...
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CD206 (MMR) Antibody: Works Out of the Box
(1)
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BioLegend
February 28, 2014
The aim of the research is to study splenic hemopoietic progenitor cells in cancer. In my experiment, I tested if a cytokine associated with...
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CD11c Antibody: Reliable
(1)
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BioLegend
February 27, 2014
The aim of the research is to study lymph node myeloid cells in cancer. In my experiment, I tested if these myeloid cells are altered in ...
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Transit-293 Transfection Reagent
(1)
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Mirus Bio
February 27, 2014
This experiment was a first-time test of the efficacy of Transit-293
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LC3B Antibody for Tissue Staining
(1)
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Cell Signaling Technology
February 26, 2014
I have tried several antibodies to stain xenograft tisse for LC3B- a marker for autophagososmes. The cell signaling LC3B D11 antibody works ...
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Excellent IL-23 ELISA Product
(1)
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BioLegend
February 25, 2014
We are trying to determine the levels of overexpressed IL-23 from the minicircle that we inject in the mice. Although the components for the...
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PrimeSTAR GXL DNA Polymerase
(1)
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February 20, 2014
For this project, my goal was to use a PCR-based strategy to genotype C. elegans strains in order to confirm the presence or absence of ...
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Source Bioscience Sanger Sequencing Service
(1)
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February 19, 2014
I have used Source Bioscience for Sanger sequencing of pre-purified PCR products. I took advantage of the free collection service and the ...
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Pacific Blue Anti-mouse F4/80
(1)
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BioLegend
February 18, 2014
The goals are to determine what are the mechanisms for inflammation-induced insulin resistance.
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MPM-2 - Cell Cycle Analysis by IF
(1)
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DAKO Corporation
February 17, 2014
MPM-2 antigens are strongly expressed within mitotic cells. In eukaryotes, MPM-2 phosphoproteins have been found in the cytoplasm of ...
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rIl-33 for in vitro and in vivo Usage
(1)
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BioLegend
February 10, 2014
We used rIl-33 from Biolegend and it works very well both in culture set up and in vivo.
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Great Cleaved Caspase 3 Antibody
(1)
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Cell Signaling Technology
February 07, 2014
Experiments were aimed at detecting apoptotic cells in postnatal mouse brain tissue. Cleaved Caspase 3 is a well-known marker for cells ...
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DNA/RNA/Protein Extraction Kit
(1)
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February 07, 2014
Macherey Nagel TriPrep kit is intended to extract DNA/RNA/protein in a single protocol. This is highly needed and desired to get consistent...
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SapphireAmp Fast PCR Master Mix
(1)
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February 06, 2014
This reagent is designed to rapidly set up a hot start PCR. This made it ideal for large scale genotyping to assess a single nucleotide ...
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Great KI67 antibody
(1)
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February 06, 2014
Used in experiments aimed at determining proliferating cell populations in the the brain of postnatal mice. KI67 is a marker for cells that ...
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Great GFAP Antibody
(1)
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DakoCytomation
February 05, 2014
Used in experiments aimed at determining astrocyte cell numbers in the brain of postnatal mice undergoing hypoxic insult. GFAP is a well-...
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Counting cells with NC3000
(1)
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February 04, 2014
We are counting our cells with NC3000 it provides cell viability, % aggregates. The instruments provides very robust counts regardless cell ...
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PCR Product Cleanup
(1)
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QIAGEN - Sample to Insight
January 31, 2014
I have used the Qiagen QIAquick PCR Purification kit to successfully purify DNA from PCR products, thus removing primers, nucleotides, ...
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Norgen Biotek Total RNA Purification Kit
(1)
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Norgen Biotek Corporation
January 30, 2014
I am interested in the microRNA expression of exosomes isolated from human plasma. I have used this kit to extract RNA from exosomes ...
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