Antibody for HA Tagging a Protein

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Quality of Results

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Cancer Research Program
Fundación IMIM
Lab manager

Company:

SIGMA

Product Name:

HA antibody

Catalog Number:

H6908

Hemagglutinin (HA) epitope tag, or HA tag, is one of the most usual ways to mark and detect a protein in vivo. Good antibody for HA tag is essential to many popular laboratory techniques as WB, IF or IP. Also transfect HA tagged protein allows to distinguish transfected from endogenous protein.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Tranfected human cells

Primary Incubation

1/2000

Blocking Agent

Milk 5% in TBS-T

Secondary Incubation

Anti Rb HRP

Tertiary Incubation

Not needed

Detection

ECL

Results Summary

Strong band at SNA1 HA and SNA2 HA fusion proteins. Softer bands probably due to degradation

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Strong and specific signal

The Bad

Any remarkable.

The Bottom Line

HA is one of the most used tag in protein detection when no protein antibody is available. SIGMA anti HA is a nice option for WB detection.

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