Good Results: Anti-SP3 and Anti-KLF6

March 20, 2014

Overall

Quality of Results

Ease-of-Optimization

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Cellular and Molecular Medicine Department
Cleveland Clinic
Project Scientist

Company:

BioLegend

Product Name:

Purified anti-SP3 and purified anti-KLF6

Catalog Number:

624801 and 642301

Purified anti-SP3 and purified anti-KLF6 antibodies give good results

Experimental Design and Results Summary

Applications

Western Blot

Sample

Hela cell lysate (30 ug total protein)

Primary Incubation

1:1000 dilution incubated at 4 degree for overnight

Blocking Agent

3% BSA

Secondary Incubation

1:5000 dilution incubated at room tempertaure for 1 hr

Tertiary Incubation

NA

Detection

ECL incubation

Results Summary

Anti-KLF6 antibody gives a single clear band at right molecular weight. Anti-SP3 gives multiple bands and the correct molecular weight band is the major signal.

Additional Notes

None

Image Gallery

Summary

The Good

The specificity and sensitivity of anti-KLF6 antibody is good. The sensitivity of anti-SP3 antibody is also OK.

The Bad

The specificity of anti-SP3 antibody is not good enough to give single band signal.

The Bottom Line

Both anti-SP3 and anti-KLF6 antibodies are good for western blot application for human cells.

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