BioLegend
PE/Cy5 anti-human CD3, PE anti-human CD4, FITC anti-human CD8a
300410, 300508, 301006
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Browse Similar Products: CD3 Flow Cytometry Antibodies
These antibodies from BioLegend work extremely well. I used these to phenotype in vitro expanded primary T cells. These antibodies give me consistent results every time. Although the CoA says use 5 ul per 50 ul reaction, I use 2.5ul per 5 ul reaction and it still works well. Because these are so cheap and work so well, I would highly recommend any budding immunologist to use them.
Flow Cytometry
In vitro expanded T cells
1:200 dilution, 20 min at 4 degC in PBS+2.5%FBS
PBS+2.5% FBS
NA
Flow cytometry - MACSQuant analyzer
I used these antibodies to phenotype in vitro expanded T cells.At day 7 post activation/expansion, >95% cells were CD3+ T cells, with a CD4:CD8 ratio of roughly 60:40.
None
Price, ease of use, specificity
Nothing
I would highly recommend using these antibodies for immunophenotyping.